DNA topoisomerase 2-binding protein 1 (Topbp1) is a 1515-residue protein from Mus musculus. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q6ZQF0.
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The mean pLDDT of this model is 65.3 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 21% |
| 70 to 90 | Confident: backbone generally right | 35% |
| 50 to 70 | Low: treat with caution | 8% |
| Below 50 | Very low: often disordered regions | 37% |
What pLDDT means and how to read it
Scaffold protein that acts as a key protein-protein adapter in DNA replication and DNA repair. Composed of multiple BRCT domains, which specifically recognize and bind phosphorylated proteins, bringing proteins together into functional combinations (PubMed:14718568). Required for DNA replication initiation but not for the formation of pre-replicative complexes or the elongation stages (By similarity). Necessary for the loading of replication factors onto chromatin, including GMNC, CDC45, DNA polymerases and components of the GINS complex (By similarity). Plays a central role in DNA repair by bridging proteins and promoting recruitment of proteins to DNA damage sites (By similarity).…
Interacts (via BRCT domains 1 and 2) with (phosphorylated) MDC1; promoting TOPBP1 recruitment to DNA damage sites during mitosis. Interacts (via BRCT domains 7 and 8) with (autophosphorylated) ATR; promoting activation of ATR. Interacts (via BRCT domains 7 and 8) with (phosphorylated) POLQ; specifically binds POLQ phosphorylated by PLK1, promoting POLQ recruitment to DNA damage sites. Interacts…
Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5U6K | X-ray | 2.6 Å | A/B/C/D/E/F/G/H=553-746 |
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