E3 ubiquitin-protein ligase Itchy homolog (ITCH) is a 903-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q96J02.
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The mean pLDDT of this model is 74.7 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 36% |
| 70 to 90 | Confident: backbone generally right | 35% |
| 50 to 70 | Low: treat with caution | 5% |
| Below 50 | Very low: often disordered regions | 23% |
What pLDDT means and how to read it
Acts as an Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11046148, PubMed:14602072, PubMed:15051726, PubMed:16387660, PubMed:17028573, PubMed:18718448, PubMed:18718449, PubMed:19116316, PubMed:19592251, PubMed:19881509, PubMed:20068034, PubMed:20392206, PubMed:20491914, PubMed:23146885, PubMed:24790097, PubMed:25631046). Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (PubMed:17028573, PubMed:18718448, PubMed:19131965, PubMed:19881509). Involved in the control of inflammatory signaling pathways…
Monomer. Part of a ternary complex composed of SMAD3, ITCH/AIP4 and NEDD9/HEF1; within the complex NEDD9/HEF1 interacts (via N-terminus) with ITCH/AIP4 (via WW domains); the complex mediates ubiquitination and proteasomal degradation of NEDD9/HEF1 (PubMed:15051726). Interacts (via WW domains) with OCNL (By similarity). Interacts (via WW domains) with NOTCH1 (By similarity). Interacts (via WW…
Cell membrane, Cytoplasm, Nucleus, Early endosome membrane, Endosome membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5CQ2 | X-ray | 1.4 Å | A=433-521 |
| 5DZD | X-ray | 1.57 Å | A/B=475-514 |
| 5DWS | X-ray | 1.65 Å | A/C/E/G=436-474 |
| 5SXP | X-ray | 1.65 Å | F/G=249-269 |
| 2NQ3 | X-ray | 1.8 Å | A=1-155 |
| 2P4R | X-ray | 2.0 Å | T=246-270 |
| 4ROF | X-ray | 2.03 Å | A/B=436-474 |
| 3TUG | X-ray | 2.27 Å | A=524-903 |
| 5C7M | X-ray | 3.03 Å | A=524-899 |
| 2DMV | NMR | A=328-357 | |
| 2KYK | NMR | A=359-392 | |
| 2YSF | NMR | A=480-512 |
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