M-phase phosphoprotein 8 (MPHOSPH8) is a 860-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q99549.
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The mean pLDDT of this model is 56.4 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 21% |
| 70 to 90 | Confident: backbone generally right | 22% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 53% |
What pLDDT means and how to read it
Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:26022416, PubMed:28581500, PubMed:29211708). As part of the HUSH complex, promotes epigenetic repression of mobile genetic elements, such as retroviruses and transposable elements: the HUSH complex mainly represses LINE-1 (L1) retrotransposons that are still capable of transposition (PubMed:29211708). Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). MPHOSPH8 mediates recruitment of the HUSH complex to…
Homodimer (PubMed:21419134, PubMed:22022377, PubMed:22086334, PubMed:39638237). Interacts (via chromo domain) with histone H3K9me3 (PubMed:20871592). Has the highest affinity for H3K9me3, and lesser affinity for H3K9me2 and H3K9me1 (PubMed:20871592). Component of the HUSH complex; composed of TASOR, PPHLN1 and MPHOSPH8 (PubMed:26022416, PubMed:39013473, PubMed:39489739). Component of the HUSH2…
Nucleus, Chromosome
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6V2S | X-ray | 1.6 Å | A/B=55-116 |
| 9H77 | X-ray | 2.01 Å | C/D=53-117 |
| 7M5U | X-ray | 2.02 Å | A=55-116 |
| 3LWE | X-ray | 2.05 Å | A/B=55-116 |
| 3R93 | X-ray | 2.06 Å | A/B/C/D=55-116 |
| 3SVM | X-ray | 2.31 Å | A=55-116 |
| 3QO2 | X-ray | 2.49 Å | A/B/C/D=55-116 |
| 8QFB | X-ray | 3.04 Å | A/B/C/D/E/F/G/H=546-860 |
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