Exoenzyme T (exoT) is a 457-residue protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9I788.
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The mean pLDDT of this model is 77.4 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 50% |
| 70 to 90 | Confident: backbone generally right | 22% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 24% |
What pLDDT means and how to read it
Bifunctional effector protein that is secreted and delivered by the type III secretion system into eukaryotic target cells. ADP-ribosylates several eukaryotic proteins including CT10 regulator of kinase (Crk) proteins (PubMed:12807879). In turn, induces atypical anoikis apoptosis by transforming Crk adaptor protein into a cytotoxin (PubMed:26020630). Affects host cell morphology by disrupting the actin cytoskeleton (PubMed:14688136). In addition to this activity, acts via its N-terminal region as a GTPase-activating protein (GAP) for host Rho GTPases including RhoA, Rac1, Cdc42 and Ras (PubMed:11895987). The bacterial Rho-GAP domain activity induces mitochondrial disruption in the target…
Interacts with chaperone protein SpcS; this interaction maintains ExoT in a secretion competent state within the cytoplasm (PubMed:24387107). Interacts with host YWHAB (PubMed:30224724)
Secreted
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 4JMF | X-ray | 2.1 Å | A=28-77 |
| 6JNP | X-ray | 2.26 Å | A/D=23-79 |
| 6GNN | X-ray | 3.79 Å | C=235-457 |
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