SR-related and CTD-associated factor 8 (SCAF8) is a 1271-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9UPN6.
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The mean pLDDT of this model is 51.4 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 17% |
| 70 to 90 | Confident: backbone generally right | 8% |
| 50 to 70 | Low: treat with caution | 10% |
| Below 50 | Very low: often disordered regions | 66% |
What pLDDT means and how to read it
Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839)
Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5' (PubMed:18550522, PubMed:31104839, PubMed:9528809). Identified in a complex with CDC5L and other spliceosomal proteins (PubMed:11101529)
Nucleus, Nucleus matrix
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3D9J | X-ray | 1.6 Å | A/B=1-136 |
| 3D9N | X-ray | 1.6 Å | A/B=1-136 |
| 3D9M | X-ray | 1.75 Å | A/B=1-136 |
| 3D9I | X-ray | 1.91 Å | A/B=1-136 |
| 3D9O | X-ray | 2.0 Å | A/B=1-136 |
| 3D9P | X-ray | 2.1 Å | A/B=1-136 |
| 3D9K | X-ray | 2.2 Å | A/B=1-136 |
| 3D9L | X-ray | 2.2 Å | A/B=1-136 |
| 2DIW | NMR | A=1-137 | |
| 9U9X | NMR | A=1-137 |
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