Microcin J25 (mcjA) is a 58-residue protein from Escherichia coli. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9X2V7.
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The mean pLDDT of this model is 49.5 (very low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 0% |
| 70 to 90 | Confident: backbone generally right | 0% |
| 50 to 70 | Low: treat with caution | 52% |
| Below 50 | Very low: often disordered regions | 48% |
What pLDDT means and how to read it
Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP) (PubMed:11443089, PubMed:12401787). Inhibits transcription by binding deep within RNAP secondary channel, where it sterically blocks the folding of the trigger loop, which is essential for efficient catalysis (PubMed:15200952, PubMed:30626643). In addition, it also seems to restrict access of nucleotide substrates to the catalytic center, and shows a partially competitive mode of inhibition with them (PubMed:15200952, PubMed:30626643). Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains…
Secreted
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 4CU4 | X-ray | 2.3 Å | B=38-58 |
| 6N60 | X-ray | 3.68 Å | M=38-58 |
| 1PP5 | NMR | A=38-58 | |
| 1Q71 | NMR | A=38-58 | |
| 1S7P | NMR | A=38-47, B=48-58 | |
| 2MMT | NMR | A=38-58 | |
| 2MMW | NMR | A=38-58 | |
| 5T56 | NMR | A/C=38-48, B/D=51-57 |
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