1AQF: Pyruvate kinase

Pyruvate kinase from rabbit muscle with MG, K, and L-phospholactate. Determined by X-ray diffraction at 2.7 Å resolution. Released 16 Sept 1998.

Method: X-ray diffraction
Resolution: 2.7 Å
Organism: Oryctolagus cuniculus
Chains: 8
Atoms: 31,410
Mol. weight: 465.86 kDa
Ligands: MG, PEQ
Released: 16 Sept 1998

Explore 1AQF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1AQF contains 199 α-helices and 210 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	17-20	4
α-helix	25-30	6
β-strand	45-49	5	1
α-helix	57-66	10
β-strand	70-74	5	1
α-helix	80-95	16
β-strand	108-112	5	1
β-strand	119	1	2
β-strand	120	1	3
β-strand	123	1	4
β-strand	126	1	4
β-strand	131-132	2	5
β-strand	138-142	5	6
α-helix	145-149	5
β-strand	151	1	4
β-strand	152	1	6
β-strand	155-157	3	6
β-strand	158	1	3
α-helix	163-166	4
β-strand	172-175	4	6
β-strand	180-187	8	6
β-strand	191-198	8	6
β-strand	201-202	2	5
β-strand	207	1	2
β-strand	208-209	2	6
α-helix	222-234	13
β-strand	238-244	7	1
α-helix	247-257	11
β-strand	265-270	6	1
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	1
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-326	4	1
α-helix	331-334	4
α-helix	341-353	13
β-strand	357-360	4	1
α-helix	362-365	4
α-helix	370-386	17
α-helix	390-400	11
α-helix	407-422	16
β-strand	426-430	5	7
α-helix	435-441	7
β-strand	449-453	5	7
α-helix	456-460	5
α-helix	461-464	4
β-strand	468-472	5	7
α-helix	481-499	19
β-strand	507-512	6	7
β-strand	523-528	6	7

Chain B: 28 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	19-21	3
α-helix	25-30	6
β-strand	45-49	5	8
α-helix	57-66	10
β-strand	70-74	5	8
α-helix	80-94	15
α-helix	95-97	3
β-strand	108-112	5	8
β-strand	119	1	9
β-strand	120	1	10
β-strand	123	1	11
β-strand	126	1	11
β-strand	132	1	12
β-strand	138-142	5	13
α-helix	145-147	3
β-strand	151	1	11
β-strand	152	1	13
β-strand	155-157	3	13
β-strand	158	1	10
α-helix	163-166	4
β-strand	172-175	4	13
β-strand	180-186	7	13
β-strand	191-197	7	13
β-strand	201	1	12
β-strand	207	1	9
β-strand	208-209	2	13
α-helix	222-233	12
β-strand	238-244	7	8
α-helix	247-257	11
β-strand	265-270	6	8
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	8
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-326	4	8
α-helix	331-334	4
α-helix	341-353	13
β-strand	357-360	4	8
α-helix	362-365	4
α-helix	370-387	18
α-helix	390-400	11
α-helix	401-403	3
α-helix	407-421	15
β-strand	427-430	4	7
α-helix	435-442	8
α-helix	448	1
β-strand	449-453	5	7
α-helix	456-461	6
α-helix	462-464	3
β-strand	468	1	7
β-strand	471-472	2	7
α-helix	476-477	2
α-helix	481-499	19
β-strand	507-512	6	7
β-strand	523-528	6	7
α-helix	529	1

Chain C: 26 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	17-20	4
α-helix	25-30	6
β-strand	45-49	5	14
α-helix	50	1
α-helix	57-66	10
β-strand	70-74	5	14
α-helix	80-94	15
α-helix	95-97	3
β-strand	108-112	5	14
β-strand	119	1	15
β-strand	120	1	16
β-strand	123	1	17
β-strand	126	1	17
β-strand	131-132	2	18
β-strand	138-142	5	19
α-helix	145-148	4
β-strand	151	1	17
β-strand	155-157	3	19
β-strand	158	1	16
α-helix	163-166	4
β-strand	172-175	4	19
β-strand	180-187	8	19
β-strand	191-197	7	19
β-strand	201-202	2	18
β-strand	207	1	15
β-strand	209	1	19
α-helix	222-234	13
β-strand	238-241	4	14
α-helix	247-257	11
β-strand	265-270	6	14
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	14
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-326	4	14
α-helix	331-334	4
α-helix	341-353	13
β-strand	357-360	4	14
α-helix	362-365	4
α-helix	370-386	17
α-helix	390-400	11
α-helix	407-421	15
β-strand	426-430	5	20
α-helix	435-441	7
β-strand	449-453	5	20
α-helix	456-461	6
α-helix	462-464	3
β-strand	468-472	5	20
α-helix	476-478	3
α-helix	481-498	18
β-strand	507-512	6	20
β-strand	523-528	6	20

Chain D: 26 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	19-21	3
α-helix	25-30	6
β-strand	45-49	5	21
α-helix	52-54	3
α-helix	57-66	10
β-strand	70-74	5	21
α-helix	80-95	16
β-strand	108-112	5	21
β-strand	119	1	22
β-strand	120	1	23
β-strand	139-142	4	24
α-helix	147-149	3
β-strand	155-157	3	24
β-strand	158	1	23
α-helix	163-166	4
β-strand	172-175	4	24
β-strand	180-187	8	24
β-strand	191-197	7	24
α-helix	206	1
β-strand	207	1	22
β-strand	208-209	2	24
α-helix	222-233	12
β-strand	238-241	4	21
α-helix	247-257	11
β-strand	265-269	5	21
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-291	4	21
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-325	3	21
α-helix	331-334	4
α-helix	341-353	13
β-strand	357-360	4	21
α-helix	362-365	4
α-helix	370-387	18
α-helix	390-401	12
α-helix	407-421	15
β-strand	427-430	4	20
α-helix	435-442	8
β-strand	449-453	5	20
α-helix	456-461	6
α-helix	462-464	3
β-strand	468-472	5	20
α-helix	476-478	3
α-helix	481-499	19
β-strand	507-512	6	20
β-strand	523-528	6	20

Chain E: 22 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	17-20	4
α-helix	25-29	5
α-helix	37-39	3
β-strand	45-50	6	25
α-helix	57-64	8
β-strand	70-74	5	25
α-helix	80-95	16
β-strand	108-112	5	25
α-helix	222-232	11
β-strand	238-241	4	25
α-helix	247-257	11
α-helix	264	1
β-strand	265-269	5	25
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	25
α-helix	293-299	7
α-helix	305-319	15
β-strand	323-326	4	25
α-helix	332-334	3
α-helix	341-353	13
β-strand	357-360	4	25
α-helix	362-365	4
α-helix	370-386	17
α-helix	390-400	11
α-helix	407-422	16
β-strand	426-430	5	26
α-helix	435-441	7
β-strand	449-453	5	26
α-helix	456-462	7
β-strand	468-472	5	26
α-helix	476-477	2
α-helix	481-499	19
β-strand	507-512	6	26
β-strand	523-528	6	26

Chain F: 24 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	17-20	4
α-helix	25-31	7
β-strand	45-49	5	27
α-helix	57-66	10
β-strand	70-74	5	27
α-helix	80-95	16
β-strand	108-112	5	27
β-strand	119	1	28
β-strand	120	1	29
β-strand	123	1	30
β-strand	126	1	30
β-strand	130-132	3	31
β-strand	140-142	3	32
α-helix	145-148	4
β-strand	151	1	30
β-strand	152	1	32
β-strand	155-157	3	32
β-strand	158	1	29
α-helix	163-166	4
β-strand	172-175	4	32
β-strand	180-187	8	32
β-strand	191-197	7	32
β-strand	201-203	3	31
α-helix	206	1
β-strand	207	1	28
β-strand	208-209	2	32
α-helix	222-233	12
β-strand	238-241	4	27
α-helix	247-257	11
β-strand	265-270	6	27
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	27
α-helix	293-299	7
α-helix	302-319	18
β-strand	323-326	4	27
α-helix	332-334	3
α-helix	341-353	13
β-strand	357-360	4	27
α-helix	362-365	4
α-helix	370-386	17
α-helix	390-399	10
α-helix	407-421	15
β-strand	426-430	5	26
α-helix	435-441	7
β-strand	449-453	5	26
α-helix	456-461	6
α-helix	462-464	3
β-strand	468-472	5	26
α-helix	476-478	3
α-helix	481-499	19
β-strand	507-512	6	26
β-strand	523-528	6	26

Chain G: 27 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	17-21	5
α-helix	25-30	6
β-strand	45-49	5	33
α-helix	50	1
α-helix	52-54	3
α-helix	57-65	9
β-strand	70-74	5	33
α-helix	80-95	16
β-strand	108-112	5	33
β-strand	119	1	34
β-strand	120	1	35
β-strand	123	1	36
β-strand	126	1	36
β-strand	131-132	2	37
α-helix	133-134	2
β-strand	138-142	5	38
β-strand	151	1	36
β-strand	152	1	38
β-strand	155-157	3	38
β-strand	158	1	35
α-helix	163-166	4
β-strand	172-175	4	38
β-strand	180-187	8	38
β-strand	192-196	5	38
β-strand	201-202	2	37
β-strand	207	1	34
β-strand	208-209	2	38
α-helix	222-232	11
β-strand	238-241	4	33
α-helix	247-257	11
α-helix	261-263	3
β-strand	265-270	6	33
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	33
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-326	4	33
α-helix	331-334	4
α-helix	341-353	13
β-strand	357-360	4	33
α-helix	362-365	4
α-helix	370-386	17
α-helix	390-400	11
α-helix	407-422	16
β-strand	427-429	3	39
α-helix	435-442	8
β-strand	449-453	5	39
α-helix	458-461	4
α-helix	462-464	3
β-strand	468-472	5	39
α-helix	476-477	2
α-helix	481-499	19
β-strand	507-512	6	39
β-strand	523-528	6	39

Chain H: 23 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	19-21	3
α-helix	25-30	6
β-strand	45	1	40
β-strand	47-49	3	40
α-helix	57-66	10
β-strand	70-74	5	40
α-helix	80-95	16
β-strand	108-112	5	40
β-strand	119	1	41
β-strand	120	1	42
β-strand	123	1	43
β-strand	126	1	43
β-strand	130-133	4	44
β-strand	138-142	5	45
α-helix	145-148	4
β-strand	151	1	43
β-strand	155-157	3	45
β-strand	158	1	42
α-helix	163-166	4
β-strand	172-175	4	45
β-strand	180-187	8	45
β-strand	191-197	7	45
β-strand	200-203	4	44
β-strand	207	1	41
β-strand	208-209	2	45
α-helix	222-233	12
β-strand	238-241	4	40
α-helix	247-257	11
β-strand	265-270	6	40
α-helix	273-277	5
α-helix	279-285	7
β-strand	288-292	5	40
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-319	15
β-strand	323-326	4	40
α-helix	331-334	4
α-helix	341-353	13
β-strand	357	1	40
α-helix	362-365	4
α-helix	370-387	18
α-helix	390-400	11
α-helix	407-421	15
β-strand	427-430	4	39
α-helix	435-442	8
β-strand	449-453	5	39
α-helix	456-461	6
α-helix	462-464	3
β-strand	468-472	5	39
α-helix	481-499	19
β-strand	507-512	6	39
β-strand	523-528	6	39

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pyruvate kinase	A, B, C, D, E, F, G, H	protein	530	Oryctolagus cuniculus	P11974 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H), FASTA

>1AQF_1 PYRUVATE KINASE (chains A, B, C, D, E, F, G, H)
SKSHSEAGSAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVETL
KEMIKSGMNVARMNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRT
GLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVDVGSKVYVDDGLI
SLQVKQKGPDFLVTEVENGGFLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVF
ASFIRKAADVHEVRKILGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEI
PAEKVFLAQKMIIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIML
SGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELARSSSHSTDLMEAMAMGSVEASY
KCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEA
WAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	8
PEQ	L-phospholactate	C3 H7 O6 P	8

Water and common crystallization additives (K) are not listed.

Primary citation

Ligand-induced domain movement in pyruvate kinase: structure of the enzyme from rabbit muscle with Mg2+, K+, and L-phospholactate at 2.7 A resolution. Larsen, T.M., Benning, M.M., Wesenberg, G.E. et al. Arch Biochem Biophys (1997) 345:199-206. DOI 10.1006/abbi.1997.0257 · PubMed

Other PDB entries of the same protein (UniProt P11974 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2G50 1.65 Å, The location of the allosteric amino acid binding site of muscle pyruvate kinase.
1A49 2.1 Å, Bis MG-ATP-K-oxalate complex of pyruvate kinase
8F6M 2.15 Å, Complex of Rabbit muscle pyruvate kinase with ADP and the phosphonate analogue of PEP…
7R6Y 2.25 Å, E117K mutant pyruvate kinase from rabbit muscle
8F5U 2.3 Å, Rabbit muscle pyruvate kinase in complex with magnesium, potassium and pyruvate
1A5U 2.35 Å, Pyruvate kinase complex with bis MG-ATP-na-oxalate
3N25 2.41 Å, The structure of muscle pyruvate kinase in complex with proline, pyruvate, and Mn2+
8F5T 2.41 Å, Rabbit muscle pyruvate kinase in complex with sodium and magnesium
1F3X 2.8 Å, S402P mutant of rabbit muscle pyruvate kinase
1PKN 2.9 Å, Structure of rabbit muscle pyruvate kinase complexed with MN2+, K+, and pyruvate
1F3W 3.0 Å, Recombinant rabbit muscle pyruvate kinase

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