1ASK: Nuclear transport factor 2 (NTF2) H66A mutant

Name: PDB 1ASK: Nuclear transport factor 2 (NTF2) H66A mutant
Published: 1998-01-28
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: transport

Nuclear transport factor 2 (NTF2) H66A mutant. Determined by X-ray diffraction at 2.3 Å resolution. Released 28 Jan 1998.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Rattus norvegicus
Chains: 2
Atoms: 2,014
Mol. weight: 28.85 kDa
Released: 28 Jan 1998

Explore 1ASK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1ASK contains 9 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-24	19
α-helix	28-32	5
β-strand	33-41	9	1
β-strand	44-47	4	1
α-helix	49-57	9
β-strand	64-75	12	1
β-strand	81-91	11	1
α-helix	95-96	2
β-strand	97-107	11	1
β-strand	112-121	10	1
α-helix	122-124	3

Chain B: 4 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-24	19
α-helix	26-32	7
β-strand	33-41	9	2
β-strand	44-47	4	2
α-helix	49-57	9
β-strand	64-75	12	2
β-strand	81-91	11	2
α-helix	95-96	2
β-strand	97-108	12	2
β-strand	111-121	11	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Nuclear transport factor 2	A, B	protein	127	Rattus norvegicus	P61972 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1ASK_1 NUCLEAR TRANSPORT FACTOR 2 (chains A, B)
MGDKPIWEQIGSSFIQHYYQLFDNDRTQLGAIYIDASCLTWEGQQFQGKAAIVEKLSSLP
FQKIQASITAQDHQPTPDSCIISMVVGQLKADEDPIMGFHQMFLLKNINDAWVCTNDMFR
LALHNFG

Primary citation

Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran. Clarkson, W.D., Corbett, A.H., Paschal, B.M. et al. J Mol Biol (1997) 272:716-730. DOI 10.1006/jmbi.1997.1255 · PubMed

Other PDB entries of the same protein (UniProt P61972 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1GY6 1.6 Å, NTF2 from rat, ammonium sulphate conditions
1JB2 2.0 Å, Crystal structure of NTF2 M84E mutant
1JB4 2.23 Å, Crystal structure of NTF2 M102E mutant
1AR0 2.3 Å, Nuclear transport factor 2 (NTF2) E42K mutant
1JB5 2.3 Å, Crystal structure of NTF2 M118E mutant
1OUN 2.3 Å, Crystal structure of nuclear transport factor 2 (NTF2)
1QMA 2.5 Å, Nuclear Transport Factor 2 (NTF2) W7A mutant
1U5O 2.5 Å, Structure of the D23A mutant of the nuclear transport carrier NTF2
5BXQ 2.5 Å, Structure of the NTF2:RanGDP complex

Browse structure collections

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