1BRN: Protein)

Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76 Å resolution. Determined by X-ray diffraction at 1.76 Å resolution. Released 31 Jan 1994.

Method: X-ray diffraction
Resolution: 1.76 Å
Organism: Bacillus amyloliquefaciens
Chains: 4
Atoms: 2,083
Mol. weight: 27.15 kDa
Released: 31 Jan 1994

Explore 1BRN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1BRN contains 8 α-helices and 14 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains L and M: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-17	11
β-strand	24-25	2	1
α-helix	27-32	6
α-helix	37-39	3
α-helix	42-45	4
β-strand	50-51	2	1
β-strand	52-56	5	2
β-strand	71-75	5	2
β-strand	87-91	5	2
β-strand	96-99	4	2
β-strand	107-108	2	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA (5'-d(cpgpapc)-3')	A, B	DNA	4
Protein (barnase (E.C.3.1.27.-))	L, M	protein	110	Bacillus amyloliquefaciens	P00648 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1BRN_1 DNA (5'-D(*CP*GP*AP*C)-3') (chains A, B)
CGAC

Sequence of entity 2 (L, M), FASTA

>1BRN_2 PROTEIN (BARNASE (E.C.3.1.27.-)) (chains L, M)
AQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNRE
GKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR

Primary citation

Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Buckle, A.M., Fersht, A.R. Biochemistry (1994) 33:1644-1653. DOI 10.1021/bi00173a005 · PubMed

Other PDB entries of the same protein (UniProt P00648 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6PQK 1.2 Å, Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A
2C4B 1.3 Å, Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase…
1A2P 1.5 Å, Barnase wildtype structure at 1.5 Å resolution
2ZA4 1.58 Å, Crystal Structural Analysis of Barnase-barstar Complex
1B20 1.7 Å, Deletion of a buried salt-bridge in barnase
1B2X 1.8 Å, Barnase wildtype structure at PH 7.5 from a cryo_cooled crystal at 100K
1B2S 1.82 Å, Structural response to mutation at a protein-protein interface
1BRI 1.9 Å, Barnase mutant with ile 76 replaced by ala
1RNB 1.9 Å, Crystal structure of a barnase-d(*gp*c) complex at 1.9 Å resolution
1X1Y 1.9 Å, Water-mediate interaction at aprotein-protein interface
3KCH 1.94 Å, Baranase crosslinked by glutaraldehyde
2F5M 1.95 Å, Cross-linked barnase soaked in bromo-ethanol

Browse structure collections

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