1FSJ: E9 DNase domain

Crystal structure of the E9 DNase domain. Determined by X-ray diffraction at 1.8 Å resolution. Released 17 Jun 2003.

Method: X-ray diffraction
Resolution: 1.8 Å
Organism: Escherichia coli
Chains: 4
Atoms: 4,546
Mol. weight: 61.12 kDa
Ligands: ZN, PO4
Released: 17 Jun 2003

Explore 1FSJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1FSJ contains 46 α-helices and 52 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 12 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3
β-strand	9-10	2	1
β-strand	12	1	2
β-strand	16	1	3
α-helix	22-25	4
β-strand	32-33	2	4
α-helix	34	1
β-strand	35	1	3
α-helix	36-42	7
β-strand	46-47	2	1
α-helix	50-63	14
α-helix	65-68	4
α-helix	73-80	8
α-helix	83-85	3
β-strand	86	1	5
α-helix	87-88	2
α-helix	89-91	3
β-strand	93	1	6
β-strand	96	1	6
β-strand	98	1	5
β-strand	100-103	4	4
α-helix	107-109	3
β-strand	115	1	2
β-strand	119-122	4	4
α-helix	124-130	7

Chain C: 12 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	209-210	2	7
β-strand	212	1	8
β-strand	216	1	9
α-helix	222-227	6
β-strand	232-233	2	10
α-helix	234	1
β-strand	235	1	9
α-helix	236-242	7
β-strand	246-247	2	7
α-helix	250-262	13
α-helix	265-268	4
α-helix	273-280	8
α-helix	283-285	3
β-strand	286	1	11
α-helix	287-288	2
α-helix	289-291	3
β-strand	293	1	12
β-strand	296	1	12
β-strand	298	1	11
β-strand	300-303	4	10
α-helix	307-309	3
β-strand	315	1	8
α-helix	316-318	3
β-strand	319-322	4	10
α-helix	324-330	7

Chain D: 12 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	404-406	3
β-strand	409-410	2	13
β-strand	412	1	14
β-strand	416	1	15
α-helix	422-427	6
β-strand	432-433	2	16
α-helix	434	1
β-strand	435	1	15
α-helix	436-442	7
β-strand	446-447	2	13
α-helix	450-462	13
α-helix	465-468	4
α-helix	473-480	8
α-helix	483-485	3
β-strand	486	1	17
α-helix	487-488	2
α-helix	489-491	3
β-strand	493	1	18
β-strand	496	1	18
β-strand	498	1	17
β-strand	500-503	4	16
α-helix	507-509	3
β-strand	515	1	14
β-strand	519-522	4	16
α-helix	524-530	7

Chain E: 10 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	604-606	3
β-strand	609-610	2	19
β-strand	612	1	20
β-strand	616	1	21
α-helix	622-625	4
β-strand	632-633	2	22
α-helix	634	1
β-strand	635	1	21
α-helix	636-642	7
β-strand	646-647	2	19
α-helix	650-663	14
α-helix	673-680	8
α-helix	683-685	3
β-strand	686	1	23
α-helix	689-691	3
β-strand	693	1	24
β-strand	696	1	24
β-strand	698	1	23
β-strand	700-703	4	22
α-helix	707-709	3
β-strand	715	1	20
β-strand	719-722	4	22
α-helix	724-730	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Colicin E9	B, C, D, E	protein	134	Escherichia coli	P09883 (AlphaFold model)

Sequence of entity 1 (B, C, D, E), FASTA

>1FSJ_1 COLICIN E9 (chains B, C, D, E)
MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEE
VSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIR
VTTPKRHIDIHRGK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4
PO4	Phosphate ion	O4 P	4

Primary citation

Structure of the E9 DNase domain in comparison with the inhibited structure of the E9 DNase/Im9 complex. Kuhlmann, U.C., Pommer, A.J., Moore, G.R. et al. To be published.

Other PDB entries of the same protein (UniProt P09883 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1FR2 1.6 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9(E41A)
2GYK 1.6 Å, Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity…
2GZJ 1.6 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)
2VLN 1.6 Å, N75A mutant of E9 DNase domain in complex with Im9
2VLQ 1.6 Å, F86A mutant of E9 DNase domain in complex with Im9
1EMV 1.7 Å, Crystal structure of colicin E9 DNase domain with its cognate immunity protein IM9 (1.7 Å)
2GZG 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (Y55F)
2GZI 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (V34A)
2GZF 1.75 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)
2WPT 1.78 Å, The crystal structure of Im2 in complex with colicin E9 DNase
2GZE 1.8 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y55A)
2VLO 1.8 Å, K97A mutant of E9 DNase domain in complex with Im9

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