1I31: Clathrin coat assembly protein AP50

MU2 adaptin subunit (AP50) of AP2 clathrin adaptor, complexed with EGFR internalization peptide fyralm at 2.5 a resolution. Determined by X-ray diffraction at 2.5 Å resolution. Released 21 Feb 2001.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Rattus norvegicus
Chains: 2
Atoms: 2,169
Mol. weight: 36.55 kDa
Released: 21 Feb 2001

Explore 1I31 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1I31 contains 5 α-helices and 18 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	172-185	14	1
β-strand	191-205	15	1
β-strand	211-216	6	2
β-strand	245-248	4	1
β-strand	253	1	2
β-strand	263-265	3	2
α-helix	267-268	2
β-strand	270-279	10	1
β-strand	287-296	10	3
β-strand	300-309	10	3
β-strand	316-325	10	1
β-strand	330-336	7	3
β-strand	341-345	5	1
α-helix	346-348	3
β-strand	350-359	10	1
β-strand	362-372	11	3
α-helix	373-374	2
α-helix	383-385	3
β-strand	386-392	7	1
β-strand	401-407	7	2
α-helix	415-417	3
β-strand	419-433	15	1

Chain P: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	3-4	2	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Clathrin coat assembly protein AP50	A	protein	314	Rattus norvegicus	P84092 (AlphaFold model)
Epidermal growth factor receptor	P	protein	6		A8IP97 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1I31_1 CLATHRIN COAT ASSEMBLY PROTEIN AP50 (chains A)
QNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESV
NLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSI
AIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKL
EVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGM
KESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKW
VRYIGRSGIYETRC

Sequence of entity 2 (P), FASTA

>1I31_2 EPIDERMAL GROWTH FACTOR RECEPTOR (chains P)
FYRALM

Primary citation

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION. Modis, Y., Boll, W., Rapoport, I. et al. To be published.

Other PDB entries of the same protein (UniProt P84092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7OIQ 1.85 Å, Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form)
7OFP 1.92 Å, Apo Structure of Mu2 Adaptin Subunit (Ap50) Of AP2 Clathrin Adaptor
7OHZ 2.27 Å, Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged)
2PR9 2.51 Å, Mu2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with GABAA…
6QH5 2.56 Å, AP2 clathrin adaptor mu2T156-phosphorylated core in closed conformation
5WRM 2.6 Å, Mu2 subunit of the clathrin adaptor complex AP2 in complex with IRS-1 Y658 peptide
2VGL 2.6 Å, AP2 clathrin adaptor core
3H85 2.6 Å, Molecular basis for the association of PIPKI gamma-p90 with the clathrin adaptor AP-2
7OI5 2.61 Å, Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved)
5WRK 2.62 Å, Mu2 subunit of the clathrin adaptor complex AP2 in complex with IRS-1 Y608 peptide
1BW8 2.65 Å, MU2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with EGFR…
1BXX 2.7 Å, MU2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with TGN38…

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