1J4L: FHA2 domain of RAD53

Solution structure of the FHA2 domain of RAD53 complexed with a phosphothreonyl peptide derived from RAD9. Determined by solution NMR. Released 5 Dec 2001.

Method: Solution NMR
Organism: Saccharomyces cerevisiae
Chains: 2
Atoms: 1,352
Mol. weight: 19.29 kDa
Released: 5 Dec 2001

Explore 1J4L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1J4L contains 1 α-helix and 15 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 15 β-strands

Element	Residues	Length	Sheet
β-strand	578-582	5	1
β-strand	592-594	3	1
β-strand	601-604	4	2
β-strand	611-612	2	2
β-strand	623-630	8	2
β-strand	644-651	8	2
β-strand	657-659	3	1
β-strand	662-664	3	1
β-strand	668-671	4	2
β-strand	676-679	4	1
β-strand	682	1	3
β-strand	689	1	3
β-strand	692-696	5	1
β-strand	702	1	2
β-strand	717-718	2	2
α-helix	721-728	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein kinase SPK1	A	protein	158	Saccharomyces cerevisiae	P22216 (AlphaFold model)
DNA repair protein RAD9	P	protein	9		P14737 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1J4L_1 PROTEIN KINASE SPK1 (chains A)
GNGRFLTLKPLPDSIIQESLEIQQGVNPFFIGRSEDCNCKIEDNRLSRVHCFIFKKRHAV
GKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLLQDGDEIKIIWDKNNKFVIGF
KVEINDTTGLFNEGLGMLQEQRVVLKQTAEEKDLVKKL

Sequence of entity 2 (P), FASTA

>1J4L_2 DNA REPAIR PROTEIN RAD9 (chains P)
EVELTQELP

Primary citation

Solution structure of the yeast Rad53 FHA2 complexed with a phosphothreonine peptide pTXXL: comparison with the structures of FHA2-pYXL and FHA1-pTXXD complexes. Byeon, I.J., Yongkiettrakul, S., Tsai, M.D. J Mol Biol (2001) 314:577-588. DOI 10.1006/jmbi.2001.5141 · PubMed

Other PDB entries of the same protein (UniProt P22216 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1G6G 1.6 Å, X-ray structure of the N-terminal fha domain from S. Cerevisiae RAD53P in complex with a…
5T2S 2.4 Å, Structure of the FHA1 domain of Rad53 bound simultaneously to the BRCT domain of Dbf4…
4PDP 2.59 Å, Crystal structure of Rad53 kinase domain and SCD2
6C4U 2.6 Å, Engineered FHA with Myc-pTBD peptide
5T2F 2.66 Å, Structure of the FHA1 domain of Rad53 bound to the BRCT domain of Dbf4
5XZW 2.8 Å, Crystal structure of Rad53 1-466
4PDS 2.9 Å, Crystal structure of Rad53 kinase domain and SCD2 in complex with AMPPNP
2YGV 2.94 Å, Conserved N-terminal domain of the yeast Histone Chaperone Asf1 in complex with the…
5XZV 3.1 Å, Crystal structure of Rad53 1-466 in complex with AMP-PNP
1DMZ A refined NMR structure of a new phophopeptide-binding domain containing the FHA2 of RAD53
1FHQ Refined solution structure of the FHA2 domain of RAD53
1FHR Solution structure of the FHA2 domain of RAD53 complexed with a phosphotyrosyl peptide

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