1K93: Adenylyl cyclase domain of anthrax edema factor

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin. Determined by X-ray diffraction at 2.95 Å resolution. Released 23 Jan 2002.

Method: X-ray diffraction
Resolution: 2.95 Å
Organisms: Bacillus anthracis, Homo sapiens
Chains: 6
Atoms: 15,241
Mol. weight: 225.75 kDa
Ligands: CA
Released: 23 Jan 2002

Explore 1K93 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1K93 contains 92 α-helices and 85 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	296-297	2	1
α-helix	298-305	8
α-helix	309-322	14
β-strand	324-328	5	1
α-helix	336-340	5
β-strand	344-345	2	2
α-helix	352-354	3
β-strand	355	1	3
β-strand	361	1	3
β-strand	363	1	2
β-strand	365	1	4
α-helix	368-370	3
α-helix	377-392	16
β-strand	398-402	5	4
β-strand	404	1	5
α-helix	407-415	9
β-strand	420-427	8	6
β-strand	430-436	7	6
β-strand	442-447	6	6
β-strand	452	1	5
β-strand	453-457	5	6
α-helix	458	1
β-strand	472-473	2	6
α-helix	474	1
β-strand	475-481	7	4
β-strand	484-487	4	4
β-strand	488-489	2	2
α-helix	492-493	2
β-strand	494-499	6	1
β-strand	500	1	7
α-helix	501-505	5
α-helix	512-517	6
α-helix	523-533	11
α-helix	534-538	5
β-strand	541-543	3	8
β-strand	547-549	3	8
α-helix	551-567	17
α-helix	580-582	3
β-strand	594-596	3	1
β-strand	602-604	3	1
α-helix	608-618	11
β-strand	624	1	7
α-helix	648-653	6
α-helix	657-659	3
α-helix	660-668	9
α-helix	696-704	9
α-helix	714-736	23
α-helix	745-767	23
α-helix	789-797	9

Chain B: 17 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	296-297	2	9
α-helix	302-305	4
α-helix	309-321	13
β-strand	324-328	5	9
α-helix	333-340	8
β-strand	344-345	2	10
β-strand	365	1	11
α-helix	368-370	3
α-helix	377-393	17
β-strand	398-402	5	11
β-strand	404	1	12
α-helix	407-415	9
β-strand	420-426	7	13
β-strand	431-436	6	13
β-strand	442-447	6	13
β-strand	452	1	12
β-strand	453-457	5	13
β-strand	472-473	2	13
β-strand	475-481	7	11
β-strand	484-487	4	11
β-strand	488-489	2	10
β-strand	494-499	6	9
β-strand	500	1	14
α-helix	501-505	5
α-helix	510-517	8
α-helix	527-533	7
α-helix	534-538	5
β-strand	541	1	15
β-strand	549	1	15
α-helix	551-568	18
β-strand	579	1	9
α-helix	580-582	3
β-strand	594-596	3	9
β-strand	602-604	3	9
α-helix	608-618	11
β-strand	624	1	14
α-helix	648-651	4
α-helix	714-728	15
α-helix	743-766	24
α-helix	786-794	9

Chain C: 24 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	296-297	2	16
α-helix	298-305	8
α-helix	309-322	14
β-strand	324-328	5	16
α-helix	336-340	5
β-strand	344-345	2	17
α-helix	352-354	3
β-strand	355	1	18
β-strand	361	1	18
β-strand	363	1	17
β-strand	365	1	19
α-helix	368-370	3
α-helix	377-393	17
β-strand	398-402	5	19
β-strand	404	1	20
α-helix	407-415	9
β-strand	420-427	8	21
β-strand	430-436	7	21
β-strand	442-447	6	21
β-strand	452	1	20
β-strand	453-457	5	21
α-helix	458	1
β-strand	472-473	2	21
α-helix	474	1
β-strand	475-481	7	19
β-strand	484-487	4	19
β-strand	488-489	2	17
α-helix	492-493	2
β-strand	494-499	6	16
β-strand	500	1	22
α-helix	501-505	5
α-helix	514-517	4
α-helix	522-533	12
α-helix	534-538	5
β-strand	541-543	3	23
β-strand	547-549	3	23
α-helix	551-567	17
α-helix	580-582	3
β-strand	594-596	3	16
β-strand	602-604	3	16
α-helix	608-618	11
β-strand	624	1	22
α-helix	648-652	5
α-helix	653-655	3
α-helix	661-669	9
α-helix	696-704	9
α-helix	714-736	23
α-helix	745-767	23
α-helix	789-797	9

Chain D: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6-15	10
β-strand	26-28	3	24
α-helix	29-38	10
α-helix	47-54	8
β-strand	62-64	3	24
α-helix	65-75	11
α-helix	79-81	3
α-helix	82-92	11
β-strand	99-100	2	25
α-helix	102-111	10
α-helix	118-128	11
β-strand	136-137	2	25
α-helix	138-145	8

Chain E: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	7-15	9
β-strand	27-28	2	26
α-helix	29-38	10
α-helix	47-54	8
β-strand	62-63	2	26
α-helix	65-75	11
α-helix	79-81	3
α-helix	82-92	11
β-strand	99-100	2	27
α-helix	102-111	10
α-helix	118-128	11
β-strand	136-137	2	27
α-helix	138-146	9

Chain F: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6-15	10
β-strand	27-28	2	28
α-helix	29-38	10
α-helix	47-54	8
β-strand	62-63	2	28
α-helix	65-75	11
α-helix	79-81	3
α-helix	82-92	11
β-strand	99-100	2	29
α-helix	102-111	10
α-helix	118-128	11
β-strand	136-137	2	29
α-helix	138-146	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calmodulin-sensitive adenylate cyclase	A, B, C	protein	510	Bacillus anthracis	P40136 (AlphaFold model)
Calmodulin	D, E, F	protein	144	Homo sapiens	P0DP23 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>1K93_1 CALMODULIN-SENSITIVE ADENYLATE CYCLASE (chains A, B, C)
DRIDVLKGEKALKASGLVPEHADAFKKIARELNTYILFRPVNKLATNLIKSGVATKGLNV
HGKSSDWGPVAGYIPFDQDLSKKHGQQLAVEKGNLENKKSITEHEGEIGKIPLKLDHLRI
EELKENGIILKGKKEIDNGKKYYLLESNNQVYEFRISDENNEVQYKTKEGKITVLGEKFN
WRNIEVMAKNVEGVLKPLTADYDLFALAPSLTEIKKQIPQKEWDKVVNTPNSLEKQKGVT
NLLIKYGIERKPDSTKGTLSNWQKQMLDRLNEAVKYTGYTGGDVVNHGTEQDNEEFPEKD
NEIFIINPEGEFILTKNWEMTGRFIEKNITGKDYLYYFNRSYNKIAPGNKAYIEWTDPIT
KAKINTIPTSAEFIKNLSSIRRSSNVGVYKDSGDKDEFAKKESVKKIAGYLSDYYNSANH
IFSQEKKRKISIFRGIQAYNEIENVLKSKQIAPEYKNYFQYLKERITNQVQLLLTHQKSN
IEFKLLYKQLNFTENETDNFEVFQKIIDEK

Sequence of entity 2 (D, E, F), FASTA

>1K93_2 CALMODULIN (chains D, E, F)
TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID
FPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEM
IREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	6

Water and common crystallization additives (SO4) are not listed.

Primary citation

Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin. Drum, C.L., Yan, S.-Z., Bard, J. et al. Nature (2002) 415:396-402. DOI 10.1038/415396a · PubMed

Other PDB entries of the same protein (UniProt P40136 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1K8T 2.6 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)
1K90 2.75 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex…
1S26 3.0 Å, Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine…
1SK6 3.2 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex…
1XFX 3.2 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the…
6UZB 3.2 Å, Anthrax toxin protective antigen channels bound to edema factor
1XFZ 3.25 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the…
1PK0 3.3 Å, Crystal Structure of the EF3-CaM complexed with PMEApp
1XFY 3.3 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin
6VRA 3.3 Å, Anthrax octamer prechannel bound to full-length edema factor
1XFU 3.35 Å, Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex…
1XFV 3.35 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP

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