1KSJ: Complex of Arl2 and PDE delta, Crystal Form 2

Complex of Arl2 and PDE delta, Crystal Form 2 (SeMet). Determined by X-ray diffraction at 2.6 Å resolution. Released 8 May 2002.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: Mus musculus, Homo sapiens
Chains: 2
Atoms: 2,694
Mol. weight: 40.36 kDa
Ligands: PO4, GTP, GDP, MG
Released: 8 May 2002

Explore 1KSJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1KSJ contains 9 α-helices and 17 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	17-23	7	1
α-helix	29-36	8
β-strand	50-57	8	1
β-strand	60-67	8	1
α-helix	71-74	4
α-helix	77-80	4
β-strand	86-92	7	1
α-helix	99-109	11
α-helix	118	1
β-strand	119-125	7	1
α-helix	135-141	7
β-strand	152-156	5	1
β-strand	158	1	2
β-strand	163	1	2
α-helix	166-178	13

Chain B: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	3-14	12
β-strand	15-24	10	1
β-strand	29-33	5	1
β-strand	45-49	5	3
α-helix	51-54	4
β-strand	59-67	9	1
β-strand	71-82	12	3
β-strand	85-97	13	3
β-strand	101-109	9	1
β-strand	127-135	9	3
β-strand	138-149	12	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
arf-like protein 2	A	protein	186	Mus musculus	Q9D0J4 (AlphaFold model)
Retinal rod rhodopsin-sensitive cgmp 3',5'-cyclic phosphodiesterase delta-subunit	B	protein	152	Homo sapiens	O43924 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1KSJ_1 arf-like protein 2 (chains A)
GSMGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDVDTISPTLGFNIKTLEHR
GFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEERLAGA
TLLIFANKQDLPGALSCNAIQEALELDSIRSHHWRIQGCSAVTGEDLLPGIDWLLDDISS
RVFTAD

Sequence of entity 2 (B), FASTA

>1KSJ_2 RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT (chains B)
GSMSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKA
VSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMM
PASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
MG	Magnesium ion	Mg	1

Water and common crystallization additives (BME) are not listed.

Primary citation

The complex of Arl2-GTP and PDE delta: from structure to function. Hanzal-Bayer, M., Renault, L., Roversi, P. et al. EMBO J (2002) 21:2095-2106. DOI 10.1093/emboj/21.9.2095 · PubMed

Other PDB entries of the same protein (UniProt Q9D0J4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1KSH 1.8 Å, Complex of Arl2 and PDE delta, Crystal Form 2 (native)
1KSG 2.3 Å, Complex of Arl2 and PDE delta, Crystal Form 1
4GOK 2.6 Å, The Crystal structure of Arl2GppNHp in complex with UNC119a

Browse structure collections

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