1MDI: Thioredoxin

High resolution solution NMR structure of mixed disulfide intermediate between mutant human thioredoxin and a 13 residue peptide comprising its target site in human nfkb. Determined by solution NMR. Released 3 Jun 1995.

Method: Solution NMR
Organism: Homo sapiens
Chains: 2
Atoms: 932
Mol. weight: 13.15 kDa
Released: 3 Jun 1995

Explore 1MDI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1MDI contains 6 α-helices and 7 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	2-5	4	1
α-helix	8-17	10
β-strand	23-27	5	1
α-helix	33-36	4
α-helix	39-42	4
α-helix	45-48	4
β-strand	53-57	5	1
α-helix	63-69	7
β-strand	74	1	2
β-strand	76-81	6	1
β-strand	84-90	7	1
α-helix	94-104	11

Chain B: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	7	1	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thioredoxin	A	protein	105	Homo sapiens	P10599 (AlphaFold model)
Target site in human nfkb	B	protein	13		P19838 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1MDI_1 THIOREDOXIN (chains A)
MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPAKMIKPFFHSLSEKYSNVIFLEVDVD
DAQDVASEAEVKATPTFQFFKKGQKVGEFSGANKEKLEATINELV

Sequence of entity 2 (B), FASTA

>1MDI_2 TARGET SITE IN HUMAN NFKB (chains B)
FRFRYVCEGPSHG

Primary citation

Solution structure of human thioredoxin in a mixed disulfide intermediate complex with its target peptide from the transcription factor NF kappa B. Qin, J., Clore, G.M., Kennedy, W.M. et al. Structure (1995) 3:289-297. DOI 10.1016/S0969-2126(01)00159-9 · PubMed

Other PDB entries of the same protein (UniProt P10599 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4OO4 0.97 Å, Crystal Structure of Human Thioredoxin Mutant
3M9J 1.1 Å, Crystal structure of human thioredoxin C69/73S double mutant, reduced form
2IFQ 1.2 Å, Crystal structure of S-nitroso thioredoxin
2HSH 1.35 Å, Crystal structure of C73S mutant of human thioredoxin-1 oxidized with H2O2
5DQY 1.4 Å, A fully oxidized human thioredoxin
3M9K 1.5 Å, Crystal structure of human thioredoxin C69/73S double-mutant, oxidized form
4OO5 1.54 Å, Crystal Structure of S-nitrosated Human Thioredoxin Mutant
1ERV 1.65 Å, Human thioredoxin mutant with cys 73 replaced by ser (reduced form)
2HXK 1.65 Å, Crystal structure of S-nitroso thioredoxin
1ERT 1.7 Å, Human thioredoxin (reduced form)
2IIY 1.7 Å, Crystal structure of S-nitroso thioredoxin
3KD0 1.7 Å, Human thioredoxin C35S,C62S,C69S,C73S mutant showing cadmium chloride bound to the…

Browse structure collections

About this viewer

MolViewer shows 1MDI directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.