1MEL: Camel single-domain vh antibody fragment

Crystal structure of a camel single-domain vh antibody fragment in complex with lysozyme. Determined by X-ray diffraction at 2.5 Å resolution. Released 16 Jun 1997.

Method: X-ray diffraction
Resolution: 2.5 Å
Organisms: Camelus dromedarius, Gallus gallus
Chains: 4
Atoms: 3,941
Mol. weight: 59.98 kDa
Released: 16 Jun 1997

Explore 1MEL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1MEL contains 20 α-helices and 40 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	10-13	4	2
β-strand	18-25	8	1
β-strand	33-39	7	2
β-strand	46-52	7	2
β-strand	58-60	3	2
β-strand	68-74	7	1
β-strand	78-83	6	1
α-helix	88-90	3
β-strand	92-99	8	2
α-helix	110-114	5
β-strand	122-123	2	2
β-strand	127-132	6	2

Chain B: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	3
β-strand	10-12	3	4
β-strand	18-25	8	3
β-strand	33-39	7	4
α-helix	44-45	2
β-strand	46-52	7	4
β-strand	58-60	3	4
β-strand	68-73	6	3
β-strand	78-83	6	3
α-helix	88-90	3
β-strand	92-99	8	4
α-helix	110-114	5
β-strand	122-123	2	4
β-strand	127-131	5	4

Chain L: 8 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	5
α-helix	5-14	10
β-strand	20	1	6
β-strand	23	1	6
α-helix	25-36	12
β-strand	39	1	5
β-strand	43-45	3	7
β-strand	51-53	3	7
β-strand	58-59	2	7
β-strand	65	1	8
β-strand	79	1	8
α-helix	80-83	4
α-helix	89-98	10
α-helix	99-103	5
α-helix	104-107	4
α-helix	109-114	6
α-helix	120-123	4

Chain M: 7 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	9
α-helix	5-14	10
β-strand	20	1	10
β-strand	23	1	10
α-helix	25-35	11
β-strand	39	1	9
β-strand	43-45	3	11
β-strand	51-53	3	11
β-strand	58-59	2	11
β-strand	65	1	12
β-strand	79	1	12
α-helix	80-84	5
α-helix	89-99	11
α-helix	104-107	4
α-helix	109-114	6
α-helix	120-123	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Vh single-domain antibody	A, B	protein	148	Camelus dromedarius
Lysozyme	L, M	protein	129	Gallus gallus	P00698 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1MEL_1 VH SINGLE-DOMAIN ANTIBODY (chains A, B)
DVQLQASGGGSVQAGGSLRLSCAASGYTIGPYCMGWFRQAPGKEREGVAAINMGGGITYY
ADSVKGRFTISQDNAKNTVYLLMNSLEPEDTAIYYCAADSTIYASYYECGHGLSTGGYGY
DSWGQGTQVTVSSGRYPYDVPDYGSGRA

Sequence of entity 2 (L, M), FASTA

>1MEL_2 LYSOZYME (chains L, M)
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
QAWIRGCRL

Primary citation

Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Desmyter, A., Transue, T.R., Ghahroudi, M.A. et al. Nat Struct Biol (1996) 3:803-811. DOI 10.1038/nsb0996-803 · PubMed

Other PDB entries of the same protein (UniProt P00698 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2VB1 0.65 Å, HEWL at 0.65 angstrom resolution
8ZST 0.8 Å, Lysozyme Crystallized with Bioassembler on Earth
9QUM 0.83 Å, Structure of lysozyme by continuous serial electron diffraction (SerialED)
7SKW 0.87 Å, Ab initio structure of triclinic lysozyme from electron-counted MicroED data
7ULY 0.87 Å, MicroED structure of triclinic lysozyme
7P6M 0.89 Å, Hydrogenated refolded hen egg-white lysozyme
9RZL 0.9 Å, Hen egg-white lysozyme (HEWL)
8RLI 0.91 Å, Neutron structure of perdeuterated hen egg-white lysozyme at room temperature
7YRK 0.92 Å, Crystal structure of the hen egg lysozyme-2-oxidobenzylidene-threoninato-copper (II)…
3LZT 0.93 Å, Refinement of triclinic lysozyme at atomic resolution
1IEE 0.94 Å, Structure of tetragonal hen egg white lysozyme at 0.94 a from crystals grown by the…
7OL5 0.94 Å, Crystal structure of Lysozyme in complex with Hepes

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1MEL is part of these collections:

Antibodies and the immune system

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