Fitting the ternary complex of ef-tu/trna/gtp and ribosomal proteins into a 13 a cryo-EM map of the coli 70S ribosome. Determined by electron microscopy at 13.0 Å resolution. Released 1 Nov 2002.
Explore 1MJ1 in 3D Show helices and sheets RCSB PDB PDBe
1MJ1 contains 18 α-helices and 25 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4 | 1 | 1 |
| β-strand | 10-17 | 8 | 2 |
| α-helix | 24-38 | 15 | |
| α-helix | 47-50 | 4 | |
| α-helix | 54-55 | 2 | |
| α-helix | 56-60 | 5 | |
| β-strand | 66-71 | 6 | 2 |
| β-strand | 75-81 | 7 | 2 |
| α-helix | 86-88 | 3 | |
| α-helix | 89-96 | 8 | |
| β-strand | 102-107 | 6 | 2 |
| α-helix | 114-125 | 12 | |
| β-strand | 131-136 | 6 | 2 |
| α-helix | 138-140 | 3 | |
| α-helix | 144-160 | 17 | |
| β-strand | 170-172 | 3 | 2 |
| α-helix | 175-185 | 11 | |
| α-helix | 194-208 | 15 | |
| α-helix | 211-213 | 3 | |
| β-strand | 216 | 1 | 1 |
| α-helix | 220-221 | 2 | |
| β-strand | 222-231 | 10 | 1 |
| β-strand | 235-244 | 10 | 1 |
| β-strand | 246-248 | 3 | 3 |
| β-strand | 252-256 | 5 | 1 |
| β-strand | 263-271 | 9 | 1 |
| β-strand | 276 | 1 | 1 |
| β-strand | 279-281 | 3 | 3 |
| β-strand | 285-290 | 6 | 1 |
| α-helix | 295-297 | 3 | |
| β-strand | 303-305 | 3 | 1 |
| β-strand | 313-322 | 10 | 4 |
| α-helix | 323-324 | 2 | |
| α-helix | 325-327 | 3 | |
| β-strand | 334 | 1 | 5 |
| β-strand | 341-344 | 4 | 4 |
| β-strand | 347-355 | 9 | 4 |
| α-helix | 356 | 1 | |
| β-strand | 362 | 1 | 5 |
| α-helix | 363 | 1 | |
| β-strand | 367-378 | 12 | 4 |
| β-strand | 385-390 | 6 | 4 |
| β-strand | 393-403 | 11 | 4 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Phe-tRNA | C, D | RNA | 76 | Escherichia coli | |
| sarcin-ricin loop of 23SrRNA | Q | RNA | 41 | Escherichia coli | |
| helix 69 of 23S rRNA | R | RNA | 27 | Escherichia coli | |
| Elongation Factor Tu | A | protein | 405 | Escherichia coli | P0CE47 (AlphaFold model) |
| S12 ribosomal protein | O | protein | 135 | Escherichia coli | Q5SHN3 (AlphaFold model) |
| S13 ribosomal protein | P | protein | 126 | Escherichia coli | P80377 (AlphaFold model) |
| L11 ribosomal protein | L | protein | 141 | Escherichia coli | P29395 (AlphaFold model) |
>1MJ1_1 Phe-tRNA (chains C, D) GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCCUGUGUUCGAUC CACAGAAUUCGCACCA
>1MJ1_2 sarcin-ricin loop of 23SrRNA (chains Q) GGGGGGCUCUUCCUAGUACGAGAGGACCGGAAGGGACGCAC
>1MJ1_3 helix 69 of 23S rRNA (chains R) GCGGCCGUAACUAUAACGGUCCUAAGG
>1MJ1_4 Elongation Factor Tu (chains A) AKGEFIRTKRHVNVGTIGHVDHGKTTLTAALTYVAAAENRNVEVKDYGDIDKAREERARG ITINTAHVEYETAKRHYSHVDCRGHADYIKNMITGAAQMDGAILVVSAADGRMRQTREHI LLARQVGVRYIVVFMNKVDMVDDRELLDLVEMEVRDLLNQYEFRGDEVRVIRGSALLALE EMHKNRKTKRGENEWVDKIWELLDAIDEYIRTRVRDVDKRFLMRVEDVFTITGRGTVATG RIERGKVKVGDEVEIVGLARETRKTVVTGVEMHRKTLQEGIAGDNVGLLLRGVSREEVER GQVLAKRGSITRHTKFEASVYILKKEEGGRHTGFFTGYRRQFYFRTTDVTGVVRLRQGVE MVMRGDNVTFTVELIKRVALEEGLRFAIREGGRTVGAGVVTKILE
>1MJ1_5 S12 ribosomal protein (chains O) MVALPTINQLVRKGREKVRKKSKVPALKGAPFRRGVCTVVRTVTPKKPNSALRKVAKVRL TSGYEVTAYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGVYDAAGVKDRKKSRSKY GTKKPKEAAKTAAKK
>1MJ1_6 S13 ribosomal protein (chains P) MARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEAEVVRLREYV ENTWKLEGELRAEVAANIKRLMDIGCYRGLRHRRGLPVRGQRTRTNARTRKGPRKTVAGK KKAPRK
>1MJ1_7 L11 ribosomal protein (chains L) MAKKVAAQIKLQLPAGKATPAPPVGPALGQHGVNIMEFCKRFNAETADKAGMILPVVITV YEDKSFTFIIKTPPASFLLKKAAGIEKGSSEPKRKIVGKVTRKQIEEIAKTKMPDLNANS LEAAMKIIEGTAKSMGIEVVD
Ribosome Interactions of Aminoacyl-tRNA and Elongation Factor TU in the Codon Recognition Complex. Stark, H., Rodnina, M.V., Wieden, H.-J. et al. Nat Struct Biol (2002) 9:849-854. PubMed
Other PDB entries of the same protein (UniProt P0CE47 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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