1N8K: Alcohol Dehydrogenase E chain

Horse Liver Alcohol Dehydrogenase Val292Thr Mutant Complexed to NAD+ and Pyrazole. Determined by X-ray diffraction at 1.13 Å resolution. Released 4 Feb 2003.

Method
X-ray diffraction
Resolution
1.13 Å
Organism
Equus caballus
Chains
2
Atoms
6,424
Mol. weight
81.55 kDa
Ligands
PZO, NAJ, ZN
Released
4 Feb 2003

Explore 1N8K in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1N8K contains 46 α-helices and 46 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand7-1481
β-strand22-2871
α-helix29-313
β-strand35-44102
α-helix47-548
β-strand63-6421
β-strand69-7682
β-strand88-9142
α-helix101-1044
β-strand130-13231
β-strand135-13841
β-strand14711
β-strand149-15352
α-helix154-1563
β-strand157-15932
α-helix166-1694
α-helix170-1734
α-helix175-1817
α-helix182-1876
β-strand194-19853
α-helix202-21312
β-strand218-22253
α-helix226-2283
α-helix229-2357
β-strand239-24133
α-helix243-2453
α-helix250-2578
β-strand26214
β-strand264-26743
α-helix272-28110
β-strand28214
β-strand288-29143
α-helix294-2963
α-helix299-3002
β-strand301-30335
α-helix306-3094
β-strand313-31643
α-helix319-3213
α-helix324-33613
α-helix343-3453
β-strand346-35162
α-helix352-3543
α-helix355-3639
β-strand369-37352
Chain B: 24 helices, 24 β-strands
ElementResiduesLengthSheet
α-helix61
β-strand7-1376
β-strand1417
α-helix20-212
β-strand22-2876
α-helix29-313
β-strand35-44108
α-helix47-548
β-strand6317
β-strand68-7698
β-strand88-9148
α-helix101-1044
β-strand130-13236
β-strand135-13736
β-strand13817
β-strand14716
β-strand149-15358
α-helix154-1563
β-strand157-15938
α-helix166-1694
α-helix170-1734
α-helix175-1817
α-helix182-1876
β-strand194-19853
α-helix202-21312
β-strand218-22253
α-helix226-2283
α-helix229-2357
β-strand239-24133
α-helix243-2453
α-helix250-2578
β-strand26219
β-strand264-26743
α-helix272-28110
β-strand28219
β-strand288-29143
α-helix294-2963
α-helix299-3002
β-strand301-30335
α-helix306-3094
β-strand313-31643
α-helix319-3213
α-helix324-33613
α-helix343-3453
β-strand346-35168
α-helix352-3543
α-helix355-3639
β-strand369-37358

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Alcohol Dehydrogenase E chainA, Bprotein374Equus caballusP00327 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>1N8K_1 Alcohol Dehydrogenase E chain (chains A, B)
STAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRSDDHVVSGTLVTP
LPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKCRVCKHPEGNFCLKNDLSMPR
GTMQDGTSRFTCRGKPIHHFLGTSTFSQYTVVDEISVAKIDAASPLEKVCLIGCGFSTGY
GSAVKVAKVTQGSTCAVFGLGGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATEC
VNPQDYKKPIQEVLTEMSNGGVDFSFEVIGRLDTMVTALSCCQEAYGVSVITGVPPDSQN
LSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVLPFEKINEGFD
LLRSGESIRTILTF

Ligands and cofactors

IDNameFormulaCopies
PZOPyrazoleC3 H4 N22
NAJNicotinamide-adenine-dinucleotide (acidic form)C21 H27 N7 O14 P22
ZNZinc ionZn4

Water and common crystallization additives (MPD) are not listed.

Primary citation

Amino Acid Residues in the Nicotinamide Binding Site Contribute to Catalysis by Horse Liver Alcohol Dehydrogenase. Rubach, J.K., Plapp, B.V. Biochemistry (2003) 42:2907-2915. DOI 10.1021/bi0272656 · PubMed

Other PDB entries of the same protein (UniProt P00327 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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