1QU2: Isoleucyl-tRNA synthetase

Insights into editing from an ile-tRNA synthetase structure with trna(ile) and mupirocin. Determined by X-ray diffraction at 2.2 Å resolution. Released 31 Aug 1999.

Method
X-ray diffraction
Resolution
2.2 Å
Organism
Staphylococcus aureus
Chains
2
Atoms
9,386
Mol. weight
130.21 kDa
Ligands
MRC, ZN, MG
Released
31 Aug 1999

Explore 1QU2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1QU2 contains 50 α-helices and 44 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 50 helices, 44 β-strands

ElementResiduesLengthSheet
α-helix4-63
α-helix20-3314
α-helix36-438
β-strand4911
β-strand52-5322
α-helix54-552
β-strand5813
α-helix65-8218
β-strand8711
β-strand93-9424
β-strand9513
α-helix99-10810
α-helix117-14024
β-strand152-15324
α-helix157-17216
β-strand176-186115
β-strand191-19225
α-helix195-1973
β-strand198-207106
β-strand208-20927
β-strand21218
β-strand221-22449
β-strand22718
β-strand230-23127
α-helix234-2363
β-strand241110
α-helix265-2728
β-strand299111
β-strand307111
β-strand326110
α-helix333-3364
α-helix344-3463
α-helix3691
α-helix370-3745
α-helix375-3773
α-helix380-3834
β-strand388-397106
β-strand40316
β-strand405-414105
α-helix416-42813
β-strand431-43222
α-helix435-44713
β-strand451-45225
β-strand454-455212
α-helix4611
β-strand462112
α-helix4631
β-strand466-467213
β-strand473-474213
α-helix477-49014
α-helix493-4975
α-helix500-5034
β-strand519-520213
β-strand524-525212
α-helix527-5326
α-helix5341
α-helix535-5406
β-strand549114
β-strand551-55552
α-helix556-5594
α-helix562-57413
β-strand579114
β-strand581-58552
β-strand588-589215
α-helix606-6127
α-helix615-6239
β-strand631-632215
α-helix635-65723
α-helix664-6674
α-helix668-6703
α-helix671-6733
α-helix676-69722
α-helix701-71010
α-helix711-72111
α-helix722-7287
α-helix736-75621
α-helix761-7688
α-helix779-7813
α-helix783-7875
α-helix792-81726
α-helix824-8263
β-strand827-832616
α-helix839-8424
α-helix843-8453
α-helix849-8524
β-strand857-860416
β-strand869-871316
β-strand874-880716
α-helix8811
β-strand884-885217
β-strand892-893217
β-strand897-899318
β-strand902-905418
α-helix907-9148

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Isoleucyl-tRNATRNA75Staphylococcus aureus
Isoleucyl-tRNA synthetaseAprotein917Staphylococcus aureusP41972 (AlphaFold model)
Sequence of entity 1 (T), FASTA
>1QU2_1 ISOLEUCYL-TRNA (chains T)
GGGCUUGUAGCUCAGGUGGUUAGAGCGCACCCCUGAUAAGGGUGAGGUCGGUGGUUCAAG
UCCACUCAGGCCCAC
Sequence of entity 2 (A), FASTA
>1QU2_2 ISOLEUCYL-TRNA SYNTHETASE (chains A)
MDYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFILHDGPPYAN
GNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIEQALTKKGVDRKKMSTAEF
REKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYEAAQIRIFGEMADKGLIYKGK
KPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDKGVVDADAKFIIWTTTPWTIPSN
VAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEWVVAQH
PFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDDYIVGQQYELPVISPIDDKGVFTEEGG
QFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTKKPVIFRATPQWFASISKVR
QDILDAIENTNFKVNWGKTRIYNMVRDRGEWVISRQRVWGVPLPVFYAENGEIIMTKETV
NHVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLE
TRPELSFPADMYLEGSDQYRGWFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSL
GNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDDYRKIRNTLRFMLGNIN
DFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFY
LDYGKDILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVH
LADMPKVVEVDQALLDKWRTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNAS
EFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVD
ELTHLCPRCQQVVKSLV

Ligands and cofactors

IDNameFormulaCopies
MRCMupirocinC26 H44 O91
ZNZinc ionZn2
MGMagnesium ionMg10

Water and common crystallization additives (K) are not listed.

Primary citation

Insights into editing from an ile-tRNA synthetase structure with tRNAile and mupirocin. Silvian, L.F., Wang, J., Steitz, T.A. Science (1999) 285:1074-1077. DOI 10.1126/science.285.5430.1074 · PubMed

Other PDB entries of the same protein (UniProt P41972 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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