1T2H: Guanyl-specific ribonuclease Sa

Y81W mutant of RNase Sa from Streptomyces aureofaciens. Determined by X-ray diffraction at 1.0 Å resolution. Released 21 Dec 2004.

Method: X-ray diffraction
Resolution: 1.0 Å
Organism: Streptomyces aureofaciens
Chains: 2
Atoms: 2,280
Mol. weight: 21.31 kDa
Released: 21 Dec 2004

Explore 1T2H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1T2H contains 9 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	1
α-helix	8-10	3
α-helix	13-23	11
α-helix	35	1
β-strand	36-37	2	1
α-helix	45-46	2
β-strand	52-56	5	1
α-helix	58-59	2
β-strand	69-73	5	1
β-strand	79-82	4	1
β-strand	90-93	4	1

Chain B: 4 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	2
α-helix	8-10	3
α-helix	13-24	12
β-strand	35-36	2	2
α-helix	37	1
α-helix	45-46	2
β-strand	53-56	4	2
β-strand	69-72	4	2
β-strand	79-82	4	2
β-strand	90-93	4	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanyl-specific ribonuclease Sa	A, B	protein	96	Streptomyces aureofaciens	P05798 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1T2H_1 Guanyl-specific ribonuclease Sa (chains A, B)
DVSGTVCLSALPPEATDTLNLIASDGPFPYSQDGVVFQNRESVLPTQSYGYYHEYTVITP
GARTRGTRRIITGEATQEDYWTGDHYATFSLIDQTC

Primary citation

Contribution of single tryptophan residues to the fluorescence and stability of ribonuclease sa. Alston, R.W., Urbanikova, L., Sevcik, J. et al. Biophys J (2004) 87:4036-4047. DOI 10.1529/biophysj.104.050377 · PubMed

Other PDB entries of the same protein (UniProt P05798 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1LNI 1.0 Å, Crystal structure analysis of a ribonuclease from streptomyces aureofaciens at atomic…
1T2I 1.1 Å, T76W mutant of RNase Sa from Streptomyces aureofaciens
4GHO 1.1 Å, Crystal Structure Analysis of Streptomyces aureofaciens Ribonuclease S24A mutant
1ZGX 1.13 Å, Crystal structure of ribonuclease mutant
1RGE 1.15 Å, Hydrolase, guanyloribonuclease
1RGF 1.2 Å, Hydrolase, guanyloribonuclease
1RGG 1.2 Å, Hydrolase, guanyloribonuclease
1RGH 1.2 Å, Hydrolase, guanyloribonuclease
1YNV 1.2 Å, Asp79 makes a large, unfavorable contribution to the stability of RNase Sa
4J5K 1.23 Å, Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa Y51F mutant
1I8V 1.25 Å, Crystal structure of RNase sa Y80F mutant
4J5G 1.31 Å, Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa T95A mutant

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