1ZL5: Botulinum neurotoxin type E

Crystal structure of Glu335Gln mutant of Clostridium botulinum neurotoxin E catalytic domain. Determined by X-ray diffraction at 2.6 Å resolution. Released 5 Jul 2005.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Clostridium botulinum
Chains: 2
Atoms: 6,575
Mol. weight: 95.66 kDa
Released: 5 Jul 2005

Explore 1ZL5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1ZL5 contains 38 α-helices and 45 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	2-4	3
α-helix	11-12	2
β-strand	17-21	5	1
β-strand	29-35	7	1
β-strand	38-41	4	1
α-helix	51-54	4
β-strand	68	1	2
α-helix	76-93	18
α-helix	97-108	12
α-helix	110-112	3
β-strand	131-134	4	1
β-strand	140-143	4	1
β-strand	147-150	4	1
β-strand	155	1	2
β-strand	160-163	4	1
β-strand	166	1	3
β-strand	170	1	3
α-helix	172-174	3
β-strand	181-184	4	1
β-strand	189-194	6	4
β-strand	200-202	3	4
α-helix	205-220	16
α-helix	224-227	4
β-strand	231-232	2	5
β-strand	246-247	2	5
α-helix	248-254	7
α-helix	256-259	4
α-helix	264-285	22
α-helix	293-295	3
α-helix	296-305	10
β-strand	308-310	3	6
β-strand	316-318	3	6
α-helix	320-331	12
α-helix	335-342	8
β-strand	355-359	5	4
β-strand	369	1	7
β-strand	373	1	7
α-helix	377-383	7
β-strand	387	1	4
α-helix	392-394	3
β-strand	395-396	2	4
α-helix	403-406	4

Chain B: 19 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	2-4	3
α-helix	11-12	2
β-strand	17-21	5	8
β-strand	29-35	7	8
β-strand	38-44	7	8
α-helix	51-53	3
α-helix	76-94	19
α-helix	97-107	11
α-helix	110-112	3
β-strand	131-134	4	8
β-strand	140-143	4	8
β-strand	147-151	5	8
β-strand	160-163	4	8
β-strand	166	1	9
β-strand	170	1	9
α-helix	172-174	3
β-strand	181-184	4	8
β-strand	189-194	6	10
β-strand	200-202	3	10
α-helix	203-204	2
α-helix	205-220	16
β-strand	231	1	11
β-strand	247	1	11
α-helix	248-254	7
α-helix	256-259	4
α-helix	264-287	24
α-helix	293-295	3
α-helix	297-305	9
β-strand	308-310	3	12
β-strand	316-318	3	12
α-helix	320-332	13
α-helix	335-342	8
β-strand	355-358	4	10
β-strand	359	1	13
β-strand	369	1	14
β-strand	373	1	14
α-helix	377-380	4
β-strand	387	1	10
α-helix	392-394	3
β-strand	395	1	13
α-helix	401-407	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
botulinum neurotoxin type E	A, B	protein	420	Clostridium botulinum	Q00496 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1ZL5_1 botulinum neurotoxin type E (chains A, B)
PKINSFNYNDPVNDRTILYIKPGGCQEFYKSFNIMKNIWIIPERNVIGTTPQDFHPPTSL
KNGDSSYYDPNYLQSDEEKDRFLKIVTKIFNRINNNLSGGILLEELSKANPYLGNDNTPD
NQFHIGDASAVEIKFSNGSQDILLPNVIIMGAEPDLFETNSSNISLRNNYMPSNHGFGSI
AIVTFSPEYSFRFNDNSMNEFIQDPALTLMHELIHSLHGLYGAKGITTKYTITQKQNPLI
TNIRGTNIEEFLTFGGTDLNIITSAQSNDIYTNLLADYKKIASKLSKVQVSNPLLNPYKD
VFEAKYGLDKDASGIYSVNINKFNDIFKKLYSFTQFDLATKFQVKCRQTYIGQYKYFKLS
NLLNDSIYNISEGYNINNLKVNFRGQNANLNPRIITPITGRGLVKKIIRFCKNIVSVKGI

Primary citation

Analysis of Active Site Residues of Botulinum Neurotoxin E by Mutational, Functional, and Structural Studies: Glu335Gln Is an Apoenzyme. Agarwal, R., Binz, T., Swaminathan, S. Biochemistry (2005) 44:8291-8302. DOI 10.1021/bi050253a · PubMed

Other PDB entries of the same protein (UniProt Q00496 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1T3C 1.9 Å, Clostridium botulinum type E catalytic domain E212Q mutant
1T3A 2.16 Å, Crystal structure of Clostridium botulinum neurotoxin type E catalytic domain
1ZKW 2.17 Å, Crystal structure of Arg347Ala mutant of botulinum neurotoxin E catalytic domain
1ZL6 2.4 Å, Crystal structure of Tyr350Ala mutant of Clostridium botulinum neurotoxin E catalytic…
7K84 2.5 Å, Crystal structure of BoNT/E LC-HN domain in complex with VHH JLE-E5
1ZKX 2.52 Å, Crystal structure of Glu158Ala/Thr159Ala/Asn160Ala- a triple mutant of Clostridium…
7UIA 2.59 Å, Crystal structure of BoNT/E receptor binding domain in complex with SV2 and VHH
1ZN3 2.6 Å, Crystal structure of Glu335Ala mutant of Clostridium botulinum neurotoxin type E
3FFZ 2.65 Å, Domain organization in Clostridium butulinum neurotoxin type E is unique: Its…
7UIB 2.77 Å, Crystal structure of BoNT/E receptor binding domain in complex with SV2, VHH, and sialic…
7UIE 3.23 Å, Crystal structure of HcE-JLE-G6
9ARJ 3.4 Å, CryoEM structure of BoNT-NTNH-OrfX2 complex from Clostridium botulinum E1, major class

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