1ZN0: Ribosome recycling factor

Coordinates of RRF and EF-G fitted into Cryo-EM map of the 50S subunit bound with both EF-G (GDPNP) and RRF. Determined by electron microscopy at 15.5 Å resolution. Released 14 Jun 2005.

Method

Electron microscopy

Resolution

15.5 Å

Organism

Escherichia coli

Chains

3

Atoms

880

Mol. weight

106.58 kDa

Released

14 Jun 2005

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Molecules and chains

>1ZN0_1 16S RIBOSOMAL RNA (chains C)
GUCACACCAUGGGAGUGGGUAUUCAUGACUGGGGUGAAGU

>1ZN0_2 Ribosome recycling factor (chains A)
MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEYYGTPTPLRQLASVTVE
DSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPLPPLTEERRKDLTKIVRGE
AEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDVQKLTDAAIKKIEAALADKEA
ELMQF

>1ZN0_3 ELONGATION FACTOR G (chains B)
EYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIAVTTCFWKDHRINIIDTPGHVDFTIE
VERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTM
QERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYH
EKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLL
DAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMADPYVGRLTFIRVYSG
TLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGED
APRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISG
MGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRQTGGRGQYGHVKIK
VEPLPRGSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSGPLIGFPVVDIKVTLYDGSYA
EVDSSEMAFKIAGSMAIKEAVQKGDPVILEPIMRVEVTTPEEYMGDVIGDLNARRGQILG
MEPRGNAQVIRAFVPLAEMFGYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLI

Primary citation

Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies. Gao, N., Zavialov, A.V., Li, W. et al. Mol Cell (2005) 18:663-674. DOI 10.1016/j.molcel.2005.05.005 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P0A805 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1ISE 2.2 Å, Crystal structure of a mutant of ribosome recycling factor from Escherichia coli,…
• 1EK8 2.3 Å, Crystal structure of the ribosome recycling factor (RRF) from escherichia coli
• 4V9D 3.0 Å, Structures of the bacterial ribosome in classical and hybrid states of tRNA binding
• 4WOI 3.0 Å, 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting…
• 4V9C 3.3 Å, Allosteric control of the ribosome by small-molecule antibiotics
• 1Y69 3.33 Å, RRF domain I in complex with the 50S ribosomal subunit from Deinococcus radiodurans
• 2RDO 9.1 Å, 50S subunit with EF-G(GDPNP) and RRF bound
• 1ZN1 14.1 Å, Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex

Browse structure collections

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