22ES: Gi bound kappa-opioid receptor
Gi bound kappa-opioid receptor in complex with difelikefalin. Determined by electron microscopy at 2.43 Å resolution. Released 6 May 2026.
- Method
- Electron microscopy
- Resolution
- 2.43 Å
- Organisms
- Rattus norvegicus, Bos taurus, Homo sapiens
- Chains
- 6
- Atoms
- 9,028
- Mol. weight
- 162.65 kDa
- Ligands
- CLR
- Released
- 6 May 2026
Explore 22ES in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
22ES contains 41 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-25 | 18 | |
| α-helix | 30-34 | 5 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 1 |
| β-strand | 58-63 | 6 | 2 |
| β-strand | 69-74 | 6 | 2 |
| β-strand | 78-83 | 6 | 2 |
| β-strand | 89-94 | 6 | 2 |
| β-strand | 100-105 | 6 | 3 |
| β-strand | 111-116 | 6 | 3 |
| β-strand | 120-125 | 6 | 3 |
| β-strand | 134-140 | 7 | 3 |
| β-strand | 146-151 | 6 | 4 |
| β-strand | 156-161 | 6 | 4 |
| β-strand | 166-170 | 5 | 4 |
| β-strand | 176-180 | 5 | 4 |
| β-strand | 187-192 | 6 | 5 |
| β-strand | 198-203 | 6 | 5 |
| β-strand | 207-212 | 6 | 5 |
| β-strand | 218-223 | 6 | 5 |
| β-strand | 229-234 | 6 | 6 |
| β-strand | 240-245 | 6 | 6 |
| β-strand | 250-254 | 5 | 6 |
| β-strand | 259-264 | 6 | 6 |
| α-helix | 272 | 1 | |
| β-strand | 273-278 | 6 | 7 |
| β-strand | 284-289 | 6 | 7 |
| β-strand | 294-298 | 5 | 7 |
| β-strand | 304-308 | 5 | 7 |
| β-strand | 315-320 | 6 | 1 |
| β-strand | 327-331 | 5 | 1 |
| β-strand | 336-339 | 4 | 1 |
Chain C: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain D: 13 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-32 | 26 | |
| β-strand | 34-39 | 6 | 10 |
| α-helix | 42-44 | 3 | |
| α-helix | 47-54 | 8 | |
| β-strand | 185-190 | 6 | 10 |
| β-strand | 195-200 | 6 | 10 |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 10 |
| α-helix | 227-230 | 4 | |
| α-helix | 232 | 1 | |
| β-strand | 233 | 1 | 11 |
| α-helix | 234 | 1 | |
| β-strand | 241 | 1 | 11 |
| α-helix | 242-254 | 13 | |
| α-helix | 257-259 | 3 | |
| β-strand | 263-269 | 7 | 10 |
| α-helix | 271-278 | 8 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-308 | 13 | |
| β-strand | 319-323 | 5 | 10 |
| α-helix | 331-351 | 21 | |
Chain E: 6 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 12 |
| β-strand | 10-12 | 3 | 13 |
| β-strand | 17-25 | 9 | 12 |
| α-helix | 29-31 | 3 | |
| β-strand | 33-39 | 7 | 13 |
| β-strand | 45-51 | 7 | 13 |
| α-helix | 53-55 | 3 | |
| β-strand | 58-60 | 3 | 13 |
| β-strand | 68-73 | 6 | 12 |
| β-strand | 78-84 | 7 | 12 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 13 |
| β-strand | 110-111 | 2 | 13 |
| β-strand | 115-119 | 5 | 13 |
| α-helix | 139-140 | 2 | |
| β-strand | 141-143 | 3 | 14 |
| β-strand | 147-149 | 3 | 15 |
| β-strand | 156-162 | 7 | 14 |
| β-strand | 167 | 1 | 16 |
| β-strand | 173 | 1 | 16 |
| β-strand | 175-180 | 6 | 15 |
| β-strand | 187-191 | 5 | 15 |
| β-strand | 195-196 | 2 | 15 |
| α-helix | 197 | 1 | |
| β-strand | 204-208 | 5 | 14 |
| β-strand | 212-217 | 6 | 14 |
| α-helix | 222-224 | 3 | |
| β-strand | 227-232 | 6 | 15 |
| β-strand | 240 | 1 | 15 |
| β-strand | 244-247 | 4 | 15 |
Chain R: 15 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 58-83 | 26 | |
| α-helix | 84-88 | 5 | |
| α-helix | 93-121 | 29 | |
| α-helix | 128-161 | 34 | |
| α-helix | 163-169 | 7 | |
| α-helix | 172-196 | 25 | |
| β-strand | 197 | 1 | 8 |
| β-strand | 200-202 | 3 | 9 |
| β-strand | 207-209 | 3 | 9 |
| α-helix | 211 | 1 | |
| β-strand | 212 | 1 | 8 |
| α-helix | 213 | 1 | |
| α-helix | 219-231 | 13 | |
| α-helix | 232-236 | 5 | |
| α-helix | 237-254 | 18 | |
| α-helix | 263-299 | 37 | |
| α-helix | 306-327 | 22 | |
| α-helix | 328-332 | 5 | |
| α-helix | 335-341 | 7 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 354 | Rattus norvegicus | P54311 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | C | protein | 71 | Bos taurus | P63212 (AlphaFold model) |
| Kappa-type opioid receptor | R | protein | 397 | Homo sapiens | P41145 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | D | protein | 354 | Homo sapiens | P63096 (AlphaFold model) |
| scFv16 | E | protein | 266 | synthetic construct | |
| Dpn-dpn-dle-dly-A1L9X | A | protein | 5 | synthetic construct | |
Sequence of entity 1 (B), FASTA
>22ES_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHHHMGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMR
TRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYA
PSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTC
ALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHE
SDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLL
AGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 2 (C), FASTA
>22ES_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 3 (R), FASTA
>22ES_3 Kappa-type opioid receptor (chains R)
DYKDDDDVDASSPIQIFRGEPGPTCAPSACLPPNSSAWFPGWAEPDSNGSAGSEDAQLEP
AHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTT
MPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRT
PLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVF
IFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFVVCWTPIH
IFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPLKMR
MERQSTSRVRNTVQDPAYLRDIDGMNKPVHHHHHHHH
Sequence of entity 4 (D), FASTA
>22ES_4 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains D)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 5 (E), FASTA
>22ES_5 scFv16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELVDENLYFQGASHHHHHHHH
Sequence of entity 6 (A), FASTA
>22ES_6 DPN-DPN-DLE-DLY-A1L9X (chains A)
FFLKX
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CLR | Cholesterol | C27 H46 O | 3 |
Primary citation
Gi bound kappa-opioid receptor in complex with difelikefalin. Zhang, H., Wang, R., Shi, P. et al. To be published.
Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
- 9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
- 7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
- 9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
- 8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
- 9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
- 8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
- 9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
- 7DUQ 2.5 Å, Cryo-EM structure of the compound 2 and GLP-1-bound human GLP-1 receptor-Gs complex
Browse structure collections
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