24TI: PCO371-bound GHRHR-Gs complex

Cryo-EM structure of the PCO371-bound GHRHR-Gs complex. Determined by electron microscopy at 2.88 Å resolution. Released 8 Jul 2026.

Method: Electron microscopy
Resolution: 2.88 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 5
Atoms: 7,970
Mol. weight: 173.25 kDa
Ligands: KHF
Released: 8 Jul 2026

Explore 24TI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

24TI contains 33 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	11-38	28
β-strand	40-47	8	1
α-helix	53-59	7
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	265-277	13
β-strand	286-292	7	1
α-helix	294-299	6
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-350	19
β-strand	359-362	4	1
α-helix	371-390	20

Chain B: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-35	6
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
α-helix	95	1
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-232	4	7
β-strand	243-245	3	7
β-strand	250-251	2	8
β-strand	254	1	9
β-strand	259	1	9
β-strand	263-264	2	8
α-helix	272	1
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	293-298	6	10
β-strand	304-309	6	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	27-29	3
α-helix	30-43	14
α-helix	53-55	3

Chain N: 1 helix, 10 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	18-25	8	11
β-strand	34-39	6	12
β-strand	46-51	6	12
β-strand	58-60	3	12
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-98	7	12
β-strand	118	1	12
β-strand	122-124	3	12

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	128-152	25
α-helix	155-157	3
α-helix	160-186	27
α-helix	200-231	32
α-helix	240-267	28
α-helix	271-273	3
α-helix	283-310	28
α-helix	325-331	7
α-helix	337-345	9
α-helix	359-361	3
α-helix	364-366	3
α-helix	368-373	6
α-helix	374-378	5
α-helix	381-393	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Growth hormone-releasing hormone receptor	R	protein	556	Homo sapiens	Q02643 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	400	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Nanobody35	N	protein	126	synthetic construct

Sequence of entity 1 (R), FASTA

>24TI_1 Growth hormone-releasing hormone receptor (chains R)
HMHPECDFITQLREDESACLQAAEEMPNTTLGCPATWDGLLCWPTAGSGEWVTLPCPDFF
SHFSSESGAVKRDCTITGWSEPFPPYPVACPVPLELLAEEESYFSTVKIIYTVGHSISIV
ALFVAITILVALRRLHCPRNYVHTQLFTTFILKAGAVFLKDAALFHSDDTDHCSFSTVLC
KVSVAASHFATMTNFSWLLAEAVYLNCLLASTSPSSRRAFWWLVLAGWGLPVLFTGTWVS
CKLAFEDIACWDLDDTSPYWWIIKGPIVLSVGVNFGLFLNIIRILVRKLEPAQGSLHTQS
QYWRLSKSTLFLIPLFGIHYIIFNFLPDNAGLGIRLPLELGLGSFQGFIVAILYCFLNQE
VRTEISRKWHGHDPELLPAWRTRGSSGGGGSGGGGSSGVFTLEDFVGDWEQTAAYNLDQV
LEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVY
PVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDER
LITPDGSMLFRVTINS

Sequence of entity 2 (A), FASTA

>24TI_2 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 3 (B), FASTA

>24TI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHSSGLVPRGSHMASHHHHHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACA
DATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYT
TNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYL
SCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDA
SAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSH
DNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMA
VATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS

Sequence of entity 4 (G), FASTA

>24TI_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>24TI_5 Nanobody35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTV

Ligands and cofactors

ID	Name	Formula	Copies
KHF	PCO-371	C29 H32 F3 N5 O6 S	1

Primary citation

Structural adaptation associated with signaling preference at the human Growth Hormone-Releasing Hormone Receptor. Wang, M.W., Hang, K., Qiu, Y. et al. Protein Cell (2026) 17:883-888. DOI 10.1093/procel/pwag016 · PubMed

Other PDB entries of the same protein (UniProt Q02643 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2XDG 1.95 Å, Crystal structure of the extracellular domain of human growth hormone releasing hormone…
7CZ5 2.6 Å, Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein…
24TB 3.03 Å, Cryo-EM structure of the ligand-free (Apo) GHRHR-Gs complex

Browse structure collections

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