2D31: Disulfide-linked HLA-G dimer

Crystal structure of disulfide-linked HLA-G dimer. Determined by X-ray diffraction at 3.2 Å resolution. Released 14 Mar 2006.

Method: X-ray diffraction
Resolution: 3.2 Å
Organism: Homo sapiens
Chains: 6
Atoms: 6,124
Mol. weight: 89.86 kDa
Released: 14 Mar 2006

Explore 2D31 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2D31 contains 19 α-helices and 59 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	4-13	10	1
α-helix	20	1
β-strand	21-28	8	1
β-strand	31-37	7	1
β-strand	46-47	2	1
α-helix	50-53	4
α-helix	57-84	28
β-strand	93-103	11	1
β-strand	109-118	10	1
β-strand	121-126	6	1
β-strand	133-135	3	1
α-helix	138-149	12
α-helix	153-161	9
α-helix	163-174	12
α-helix	176-180	5
α-helix	182	1
β-strand	183	1	2
α-helix	184-185	2
β-strand	188-190	3	3
β-strand	200-208	9	3
β-strand	209	1	2
β-strand	214-218	5	4
β-strand	223	1	4
β-strand	229-230	2	3
α-helix	231-233	3
β-strand	234-235	2	3
β-strand	241-248	8	3
β-strand	258-262	5	4
β-strand	271-272	2	4

Chain B: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	5
β-strand	6-11	6	6
β-strand	21-30	10	6
β-strand	31	1	5
β-strand	35-41	7	7
β-strand	44-45	2	7
α-helix	46	1
β-strand	50-51	2	6
β-strand	55-56	2	6
β-strand	62-70	9	6
β-strand	78-84	7	7
β-strand	91-94	4	7

Chain D: 8 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	4-14	11	8
β-strand	18-28	11	8
β-strand	31-37	7	8
β-strand	46-47	2	8
α-helix	50-52	3
α-helix	57-84	28
β-strand	94-103	10	8
β-strand	109-118	10	8
β-strand	121-126	6	8
β-strand	133-135	3	8
α-helix	138-149	12
α-helix	152-158	7
α-helix	159-164	6
α-helix	165-174	10
α-helix	176-179	4
β-strand	183	1	9
β-strand	186-190	5	10
β-strand	202-208	7	10
β-strand	209	1	9
β-strand	229-230	2	10
α-helix	231-233	3
β-strand	234-235	2	10
β-strand	241-246	6	10
β-strand	258-261	4	11
β-strand	270-273	4	11

Chain E: 0 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	12
β-strand	6	1	13
β-strand	9-11	3	14
β-strand	21-30	10	14
β-strand	31	1	12
β-strand	36-41	6	15
β-strand	45	1	15
β-strand	50	1	14
β-strand	55-56	2	14
β-strand	62-70	9	14
β-strand	78-83	6	15
β-strand	91-94	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
HLA class I histocompatibility antigen, alpha chain G	A, D	protein	276	Homo sapiens	P17693 (AlphaFold model)
Beta-2-microglobulin	B, E	protein	100	Homo sapiens	P61769 (AlphaFold model)
9-mer peptide from Histone H2A	C, F	protein	9	Homo sapiens	P16104 (AlphaFold model)

Sequence of entity 1 (A, D), FASTA

>2D31_1 HLA class I histocompatibility antigen, alpha chain G (chains A, D)
GSHSMRYFSAAVSRPGRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEQEGPEYW
EEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDG
KDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQ
RADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETRPAGDGT
FQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQ

Sequence of entity 2 (B, E), FASTA

>2D31_2 Beta-2-microglobulin (chains B, E)
MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKD
WSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Sequence of entity 3 (C, F), FASTA

>2D31_3 9-mer peptide from Histone H2A (chains C, F)
RIIPRHLQL

Primary citation

Efficient Leukocyte Ig-like Receptor Signaling and Crystal Structure of Disulfide-linked HLA-G Dimer. Shiroishi, M., Kuroki, K., Ose, T. et al. J Biol Chem (2006) 281:10439-10447. DOI 10.1074/jbc.M512305200 · PubMed

Other PDB entries of the same protein (UniProt P17693 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3KYO 1.7 Å, Crystal structure of HLA-G presenting KLPAQFYIL peptide
1YDP 1.9 Å, 1.9A crystal structure of HLA-G
3KYN 2.4 Å, Crystal structure of HLA-G presenting KGPPAALTL peptide
2DYP 2.5 Å, Crystal Structure of LILRB2(LIR2/ILT4/CD85d) complexed with HLA-G
3BZE 2.5 Å, The human non-classical major histocompatibility complex molecule HLA-E
6K60 3.15 Å, Structural and functional basis for HLA-G isoform recognition of immune checkpoint…
6AEE 3.3 Å, Crystal structure of the four Ig-like domains of LILRB1 complexed with HLA-G
3CDG 3.4 Å, Human CD94/NKG2A in complex with HLA-E
9RWI 3.45 Å, crystal structure of a human leukocyte antigen-A (HLA-G) blocking antibody
3CII 4.41 Å, Structure of NKG2A/CD94 bound to HLA-E

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