9RWI: Histone H2AX

crystal structure of a human leukocyte antigen-A (HLA-G) blocking antibody. Determined by X-ray diffraction at 3.45 Å resolution. Released 22 Jul 2026.

Method: X-ray diffraction
Resolution: 3.45 Å
Organisms: Homo sapiens, Oryctolagus cuniculus
Chains: 5
Atoms: 6,214
Mol. weight: 89.24 kDa
Released: 22 Jul 2026

Explore 9RWI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9RWI contains 21 α-helices and 77 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	27-36	10	1
β-strand	45-52	8	1
β-strand	55-61	7	1
β-strand	70-71	2	1
α-helix	81-108	28
β-strand	118-127	10	1
β-strand	133-142	10	1
β-strand	146-150	5	1
β-strand	157-159	3	1
α-helix	172-174	3
α-helix	176-182	7
α-helix	183-187	5
α-helix	188-198	11
α-helix	200-203	4
β-strand	207	1	2
α-helix	208-209	2
β-strand	213-217	5	3
β-strand	222-227	6	3
β-strand	232	1	4
β-strand	233	1	2
β-strand	237-243	7	5
β-strand	247	1	5
β-strand	252-254	3	3
α-helix	255-257	3
β-strand	258-259	2	4
β-strand	265-266	2	4
β-strand	269-274	6	3
α-helix	278-280	3
β-strand	281-287	7	5
β-strand	294-296	3	5

Chain B: 1 helix, 8 β-strands

Element	Residues	Length	Sheet
α-helix	23-25	3
β-strand	26-31	6	6
β-strand	42-50	9	6
β-strand	56-61	6	7
β-strand	70	1	6
β-strand	75-76	2	6
β-strand	82-89	8	6
β-strand	98-103	6	7
β-strand	112-113	2	7

Chain H: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	8
β-strand	9-11	3	9
β-strand	17	1	10
β-strand	20-24	5	8
β-strand	33-38	6	9
β-strand	44-50	7	9
β-strand	56-58	3	9
β-strand	63	1	11
β-strand	66	1	11
β-strand	69-71	3	8
α-helix	72-74	3
β-strand	76-78	3	8
β-strand	81	1	10
β-strand	88-94	7	9
β-strand	103	1	9
β-strand	108-112	5	9
α-helix	115-117	3
β-strand	118	1	12
α-helix	119-120	2
β-strand	121-125	5	13
β-strand	138-146	9	13
β-strand	147	1	12
β-strand	152-155	4	14
β-strand	160	1	14
β-strand	164-166	3	13
α-helix	167-169	3
β-strand	170-171	2	13
β-strand	177-184	8	13
β-strand	192-198	7	14
α-helix	199-201	3
β-strand	203-209	7	14

Chain L: 6 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	15
β-strand	10-14	5	16
β-strand	18	1	17
β-strand	21-25	5	15
β-strand	33-38	6	18
α-helix	43-44	2
β-strand	45-49	5	18
β-strand	53-54	2	18
β-strand	63-66	4	15
β-strand	70-74	5	15
β-strand	76	1	17
α-helix	80-82	3
β-strand	85-90	6	18
β-strand	101	1	18
β-strand	105	1	18
β-strand	106-110	5	16
β-strand	118-121	4	19
α-helix	122-124	3
α-helix	127-130	4
β-strand	132-142	11	19
β-strand	147-152	6	20
β-strand	155-156	2	20
α-helix	157	1
β-strand	161-165	5	19
α-helix	166-169	4
β-strand	175-184	10	19
β-strand	194-200	7	20
β-strand	203-209	7	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Histone H2AX	P	protein	8	Homo sapiens	P16104 (AlphaFold model)
HLA class I histocompatibility antigen, alpha chain G	A	protein	275	Homo sapiens	P17693 (AlphaFold model)
Beta-2-microglobulin	B	protein	99	Homo sapiens	P61769 (AlphaFold model)
Fab heavy chain	H	protein	212	Oryctolagus cuniculus
Fab light chain	L	protein	213	Oryctolagus cuniculus

Sequence of entity 1 (P), FASTA

>9RWI_1 Histone H2AX (chains P)
IIPRHLQL

Sequence of entity 2 (A), FASTA

>9RWI_2 HLA class I histocompatibility antigen, alpha chain G (chains A)
SHSMRYFSAAVSRPGRGEPRFIAMGYVDDTQFVRFDSDSASPRMEPRAPWVEQEGPEYWE
EETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGK
DYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQR
ADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETRPAGDGTF
QKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQ

Sequence of entity 3 (B), FASTA

>9RWI_3 Beta-2-microglobulin (chains B)
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW
SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Sequence of entity 4 (H), FASTA

>9RWI_4 Fab heavy chain (chains H)
QSVEESGGRLVTPGTPLTLTCTVSGIDLSSNAMSWVRQAPGEGLEWIGTISSGGRTYYAS
WAKGRFTISKTSTTVDLKIPSPTTEDTATYFCGRGDGATGFNIWGPGTLVTVSSGQPKAP
SVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLS
SVVSVTSSSQPVTCNVAHPATNTKVDKTVAPS

Sequence of entity 5 (L), FASTA

>9RWI_5 Fab light chain (chains L)
ALVMTQTPASVSEPVGGTVTIKCQASQSIYSYLSWYQQKPGQPPKLLIYKASTLASGVSS
RFKGSGSGTQFTLTISDLECGDAATYYCQNHWNVGGNGWPFGGGTEVVVKRTPVAPTVLI
FPPAADQVATGTVTIVCVANKYYPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSST
LTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGD

Primary citation

Preclinical characterisation and activity of a human leukocyte antigen-A (HLA-G) blocking antibody with enhanced Fc receptor-mediated effector function. O Dowd, V., McElhone, R., Thompson, C. et al. To be published.

Other PDB entries of the same protein (UniProt P16104 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3U3Z 1.5 Å, Structure of human microcephalin (MCPH1) tandem BRCT domains in complex with an H2A.X…
6ZWK 1.55 Å, Crystal structure of the phosphorylated C-terminal tail of histone H2AX in complex with…
1YDP 1.9 Å, 1.9A crystal structure of HLA-G
3SHV 2.1 Å, Crystal structure of human MCPH1 tandem BRCT domains-gamma H2AX complex
3SQD 2.15 Å, Crystal structure of human PTIP BRCT5/6-gamma H2AX complex
6K1K 2.2 Å, Human nucleosome core particle with H2A.X S139E variant
2AZM 2.41 Å, Crystal structure of the MDC1 brct repeat in complex with the histone tail of gamma-H2AX
2DYP 2.5 Å, Crystal Structure of LILRB2(LIR2/ILT4/CD85d) complexed with HLA-G
3SZM 2.63 Å, Structure of human microcephalin (MCPH1) tandem brct domains in complex with a…
6K1I 2.75 Å, Human nucleosome core particle with gammaH2A.X variant
6K1J 2.85 Å, Human nucleosome core particle with H2A.X variant
9Q8X 2.94 Å, Ku70/80 bound to a 153 bp H2AX nucleosome

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