2E43: C/EBPbeta Bzip homodimer K269A mutant

Crystal structure of C/EBPbeta Bzip homodimer K269A mutant bound to A High Affinity DNA fragment. Determined by X-ray diffraction at 2.1 Å resolution. Released 18 Dec 2007.

Method: X-ray diffraction
Resolution: 2.1 Å
Organism: Homo sapiens
Chains: 4
Atoms: 2,062
Mol. weight: 28.44 kDa
Released: 18 Dec 2007

Explore 2E43 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2E43 contains 2 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	272-329	58

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	272-332	61

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA (5'-d(pdtpdapdgpdgpdapdtpdtpdgpdcpdgpdcpdapdapdtpdapdt)-3')	C	DNA	16
DNA (5'-d(pdapdapdtpdapdtpdtpdgpdcpdgpdcpdapdapdtpdcpdcpdt)-3')	D	DNA	16
CCAAT/enhancer-binding protein beta	A, B	protein	78	Homo sapiens	P17676 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>2E43_1 DNA (5'-D(P*DTP*DAP*DGP*DGP*DAP*DTP*DTP*DGP*DCP*DGP*DCP*DAP*DAP*DTP*DAP*DT)-3') (chains C)
TAGGATTGCGCAATAT

Sequence of entity 2 (D), FASTA

>2E43_2 DNA (5'-D(P*DAP*DAP*DTP*DAP*DTP*DTP*DGP*DCP*DGP*DCP*DAP*DAP*DTP*DCP*DCP*DT)-3') (chains D)
AATATTGCGCAATCCT

Sequence of entity 3 (A, B), FASTA

>2E43_3 CCAAT/enhancer-binding protein beta (chains A, B)
VKSKAKKTVDAHSDEYKIRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENERLQKKVE
QLSRELSTLRNLFKQLPE

Primary citation

Structural Basis for Flexible Base Recognition by C/Ebpbeta. Tahirov, T.H., Inoue-Bungo, T., Sato, K. et al. To be published.

Other PDB entries of the same protein (UniProt P17676 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6MG1 1.75 Å, C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide…
7L4V 1.75 Å, C-terminal bZIP domain of human C/EBPbeta Bound to DNA with Consensus Recognition with…
1GU4 1.8 Å, Crystal structure of C/EBPBETA BZIP homodimer bound to a high affinity DNA fragment
2E42 1.8 Å, Crystal structure of C/EBPbeta Bzip homodimer V285A mutant bound to A High Affinity DNA…
1GTW 1.85 Å, crystal structure of C/EBPbeta bZip homodimer bound to a DNA fragment from the tom-1A…
6MG2 1.93 Å, C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide…
6MG3 2.05 Å, V285A Mutant of the C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated…
1GU5 2.1 Å, Crystal structure of C/EBPBETA BZIP homodimer bound to a DNA fragment from the MIM-1…
8K8D 2.2 Å, Crystal structure of C/EBPbeta BZIP domain bound to a high affinity DNA
1H8A 2.23 Å, Crystal structure of ternary protein-DNA COMPLEX3
1H89 2.45 Å, Crystal structure of ternary protein-DNA COMPLEX2
7UPZ 2.49 Å, Structural basis for cell type specific DNA binding of C/EBPbeta: the case of cell cycle…

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