2JRI: Josephin domain of Ataxin-3

Solution structure of the Josephin domain of Ataxin-3 in complex with ubiquitin molecule. Determined by solution NMR. Released 1 Jul 2008.

Method: Solution NMR
Organism: Homo sapiens
Chains: 3
Atoms: 2,683
Mol. weight: 38.21 kDa
Released: 1 Jul 2008

Explore 2JRI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2JRI contains 15 α-helices and 24 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	14-20	7
α-helix	32-50	19
α-helix	57-60	4
β-strand	66	1	1
β-strand	69	1	1
α-helix	77-87	11
β-strand	90-93	4	2
α-helix	97-100	4
β-strand	111-115	5	2
β-strand	120-126	7	2
β-strand	129-133	5	2
β-strand	141-143	3	2
α-helix	145-158	14
β-strand	161-166	6	2
α-helix	173-177	5
α-helix	178-180	3

Chain B: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	184-189	6	3
β-strand	193-198	6	3
β-strand	204	1	4
α-helix	205-215	11
α-helix	220-222	3
β-strand	224-227	4	3
β-strand	230-231	2	3
α-helix	232-233	2
β-strand	237	1	4
α-helix	239-241	3
β-strand	248-252	5	3

Chain C: 3 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	260	1	5
β-strand	264	1	6
β-strand	270	1	6
β-strand	274	1	5
β-strand	280	1	7
α-helix	281-291	11
β-strand	300-302	3	6
β-strand	307	1	6
α-helix	308-309	2
β-strand	313	1	7
α-helix	314-317	4
β-strand	326-328	3	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ataxin-3	A	protein	182	Homo sapiens	P54252 (AlphaFold model)
UBC protein	B, C	protein	76	Homo sapiens	P0CG48 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2JRI_1 Ataxin-3 (chains A)
MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRT
FLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHW
FTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQM
IR

Sequence of entity 2 (B, C), FASTA

>2JRI_2 UBC protein (chains B, C)
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG

Primary citation

Understanding the plasticity of the ubiquitin-protein recognition code: the josephin domain of ataxin-3 is a diubiquitin binding motif. Nicastro, G., Menon, R., Masino, L. et al. To be published.

Other PDB entries of the same protein (UniProt P54252 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4YS9 2.0 Å, Ataxin-3 Carboxy-Terminal Region - Crystal C1 (tetragonal)
4WTH 2.25 Å, Ataxin-3 Carboxy Terminal Region - Crystal C2 (triclinic)
1YZB Solution structure of the Josephin domain of Ataxin-3
2AGA De-ubiquitinating function of ataxin-3: insights from the solution structure of the…
2DOS Structural basis for the recognition of Lys48-linked polyubiquitin chain by the Josephin…
2KLZ Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin

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