2KJH: Ubiquitin/ISG15-conjugating enzyme E2 L6

NMR based structural model of the UBCH8-UBIQUITIN complex. Determined by solution NMR. Released 8 Dec 2009.

Method: Solution NMR
Organism: Homo sapiens
Chains: 2
Atoms: 1,831
Mol. weight: 26.28 kDa
Released: 8 Dec 2009

Explore 2KJH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2KJH contains 9 α-helices and 15 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	3-13	11
β-strand	21-27	7	1
β-strand	30-38	9	1
α-helix	45-47	3
β-strand	48-49	2	2
β-strand	50-55	6	1
β-strand	66-69	4	1
β-strand	78	1	3
β-strand	84	1	3
α-helix	87-89	3
α-helix	100-112	13
α-helix	122-128	7
α-helix	132-143	12
β-strand	148-149	2	2
α-helix	150	1

Chain B: 2 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	4
β-strand	12-16	5	4
β-strand	22	1	5
α-helix	23-33	11
α-helix	38-40	3
β-strand	41-45	5	4
β-strand	48-49	2	4
β-strand	55	1	5
β-strand	66-71	6	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ubiquitin/ISG15-conjugating enzyme E2 L6	A	protein	152	Homo sapiens	O14933 (AlphaFold model)
Ubiquitin	B	protein	76	Homo sapiens	P0CG48 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2KJH_1 Ubiquitin/ISG15-conjugating enzyme E2 L6 (chains A)
MASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEY
PFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPL
RMDLADLLTQNPELFRKNAEEFTLRFGVDRPS

Sequence of entity 2 (B), FASTA

>2KJH_2 Ubiquitin (chains B)
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGC

Primary citation

The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site. Serniwka, S.A., Shaw, G.S. Biochemistry (2009) 48:12169-12179. DOI 10.1021/bi901686j · PubMed

Other PDB entries of the same protein (UniProt O14933 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1WZV 2.1 Å, Crystal Structure of UbcH8
1WZW 2.4 Å, Crystal Structure of UbcH8
9LW4 2.7 Å, UbCh8-ISG15 complex
8SE9 3.2 Å, Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex…
8SEB 3.24 Å, Cryo-EM structure of a single loaded human UBA7-UBE2L6-ISG15 adenylate complex
8SV8 3.38 Å, Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex…
8SEA 3.4 Å, Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex…
8OIF 3.5 Å, Structure of the UBE1L activating enzyme bound to ISG15 and UBE2L6

Browse structure collections

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