2N5T: Phosphoenolpyruvate-protein phosphotransferase

Ensemble solution structure of the phosphoenolpyruvate-Enzyme I complex from the bacterial phosphotransferase system. Determined by solution NMR. Released 2 Sept 2015.

Method
Solution NMR
Organism
Escherichia coli
Chains
2
Atoms
8,882
Mol. weight
127.27 kDa
Released
2 Sept 2015

Explore 2N5T in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2N5T contains 59 α-helices and 34 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 30 helices, 17 β-strands

ElementResiduesLengthSheet
β-strand811
β-strand12-1871
α-helix30-323
α-helix33-6432
α-helix67-8014
α-helix83-919
α-helix92-965
α-helix100-11617
α-helix121-14222
β-strand156-16051
α-helix165-1695
β-strand176-18161
α-helix189-1979
β-strand201-20221
β-strand216-22051
β-strand227-22931
α-helix233-25321
β-strand26212
β-strand26812
β-strand270-27563
α-helix279-2868
β-strand292-29653
α-helix297-3015
α-helix310-32314
β-strand329-33243
α-helix333-3342
α-helix343-3453
α-helix347-3493
α-helix353-3553
α-helix360-3634
α-helix367-38014
β-strand386-39053
α-helix396-41520
β-strand425-43063
α-helix433-4375
α-helix439-4435
β-strand448-45143
α-helix453-4608
α-helix468-4736
α-helix479-49416
β-strand498-50143
α-helix512-5176
β-strand522-52543
α-helix527-5293
α-helix530-5389
α-helix542-55413
α-helix558-57215
Chain B: 29 helices, 17 β-strands
ElementResiduesLengthSheet
β-strand814
β-strand12-1874
α-helix33-6432
α-helix67-8014
α-helix83-919
α-helix92-965
α-helix100-11617
α-helix121-14222
β-strand156-16054
α-helix165-1695
β-strand176-18164
α-helix189-1979
β-strand201-20224
β-strand216-22054
β-strand227-22934
α-helix233-25321
β-strand26215
β-strand26815
β-strand270-27566
α-helix279-2868
β-strand292-29656
α-helix297-3015
α-helix310-32314
β-strand329-33246
α-helix333-3342
α-helix343-3453
α-helix347-3493
α-helix353-3553
α-helix360-3634
α-helix367-38014
β-strand386-39056
α-helix396-41520
β-strand425-43066
α-helix433-4375
α-helix439-4435
β-strand448-45146
α-helix453-4608
α-helix468-4736
α-helix479-49416
β-strand498-50146
α-helix512-5176
β-strand522-52546
α-helix527-5293
α-helix530-5389
α-helix542-55413
α-helix558-57215

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Phosphoenolpyruvate-protein phosphotransferaseA, Bprotein575Escherichia coliP08839 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>2N5T_1 Phosphoenolpyruvate-protein phosphotransferase (chains A, B)
MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFLSGRAKASAQLETIKTK
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQASALEELDD
EYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVAADLTPSETAQLNLKKVLGFI
TDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYLILDAVNNQVYVNPTNEVIDKMR
AVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL
FMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAI
RIAMDRREILRDQLRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELRDEGKAF
DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
NFEDAKVLAEQALAQPTTDELMTLVNKFIEEKTIC

Primary citation

Dynamic equilibrium between closed and partially closed states of the bacterial Enzyme I unveiled by solution NMR and X-ray scattering. Venditti, V., Schwieters, C.D., Grishaev, A. et al. Proc Natl Acad Sci U S A (2015) 112:11565-11570. DOI 10.1073/pnas.1515366112 · PubMed

Other PDB entries of the same protein (UniProt P08839 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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