2OUG: RfaH transcription factor

Name: PDB 2OUG: Crystal structure of the RfaH transcription factor at 2.1A resolution
Published: 2007-05-01
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: transcription

Crystal structure of the RfaH transcription factor at 2.1A resolution. Determined by X-ray diffraction at 2.1 Å resolution. Released 1 May 2007.

Method: X-ray diffraction
Resolution: 2.1 Å
Organism: Escherichia coli
Chains: 4
Atoms: 4,981
Mol. weight: 73.46 kDa
Released: 1 May 2007

Explore 2OUG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2OUG contains 24 α-helices and 26 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	1
α-helix	14-23	10
β-strand	27-29	3	1
β-strand	32-38	7	2
β-strand	43-49	7	2
β-strand	54-59	6	1
α-helix	66-71	6
β-strand	75-78	4	1
α-helix	86-87	2
α-helix	91-98	8
α-helix	118-129	12
α-helix	135-155	21

Chain B: 6 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	3
α-helix	14-23	10
β-strand	27-29	3	3
β-strand	32-38	7	4
β-strand	43-49	7	4
β-strand	54-59	6	3
α-helix	66-71	6
β-strand	75-78	4	3
β-strand	80	1	5
β-strand	86	1	5
α-helix	87	1
α-helix	91-98	8
α-helix	119-129	11
α-helix	135-155	21

Chain C: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	6
α-helix	14-23	10
β-strand	27-29	3	6
β-strand	32-38	7	7
β-strand	43-49	7	7
β-strand	54-59	6	6
α-helix	66-71	6
β-strand	75-78	4	6
α-helix	86-87	2
α-helix	91-98	8
α-helix	119-129	11
α-helix	135-155	21

Chain D: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	8
α-helix	14-23	10
β-strand	27-29	3	8
β-strand	32-36	5	9
β-strand	45-49	5	9
β-strand	54-59	6	8
α-helix	66-71	6
β-strand	75-78	4	8
α-helix	86-87	2
α-helix	91-98	8
α-helix	119-129	11
α-helix	135-155	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transcriptional activator rfaH	A, B, C, D	protein	162	Escherichia coli	P0AFW0 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>2OUG_1 Transcriptional activator rfaH (chains A, B, C, D)
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFD
PEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITE
GAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL

Primary citation

Structural basis for converting a general transcription factor into an operon-specific virulence regulator. Belogurov, G.A., Vassylyeva, M.N., Svetlov, V. et al. Mol Cell (2007) 26:117-129. DOI 10.1016/j.molcel.2007.02.021 · PubMed

Other PDB entries of the same protein (UniProt P0AFW0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5OND 2.1 Å, RfaH from Escherichia coli in complex with ops DNA
8PIB 2.6 Å, autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter…
8PID 3.0 Å, backtracked E. coli transcription complex paused at ops site and bound to RfaH
8PEN 3.1 Å, fully recruited RfaH bound to E. coli transcription complex paused at ops site…
8PFG 3.1 Å, autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter…
8PHK 3.1 Å, fully recruited RfaH bound to E. coli transcription complex paused at ops site
8URY 3.1 Å, Escherichia coli transcription-translation coupled complex class B (TTC-B) containing…
8PIL 3.2 Å, E. coli transcription complex paused at ops site and bound to RfaH and NusA
8UQL 3.2 Å, Escherichia coli transcription-translation coupled complex class B (TTC-B) containing…
8PFJ 3.4 Å, fully recruited RfaH bound to E. coli transcription complex paused at ops site (not…
8PIM 3.4 Å, fully recruited RfaH bound to E. coli transcription complex paused at ops site (not…
8UR0 3.4 Å, Escherichia coli transcription-translation coupled complex class B (TTC-B) containing…

Browse structure collections

About this viewer

MolViewer shows 2OUG directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.