2PF4: Full-length simian virus 40 small t antigen
Crystal structure of the full-length simian virus 40 small t antigen complexed with the protein phosphatase 2A Aalpha subunit. Determined by X-ray diffraction at 3.1 Å resolution. Released 7 Aug 2007.
- Method
- X-ray diffraction
- Resolution
- 3.1 Å
- Organisms
- Mus musculus, Simian virus 40
- Chains
- 8
- Atoms
- 22,802
- Mol. weight
- 344.17 kDa
- Ligands
- ZN
- Released
- 7 Aug 2007
Explore 2PF4 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
2PF4 contains 269 α-helices and 8 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 60 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 35-41 | 7 | |
| α-helix | 43-46 | 4 | |
| α-helix | 47-51 | 5 | |
| α-helix | 52-57 | 6 | |
| α-helix | 65-73 | 9 | |
| α-helix | 77-79 | 3 | |
| α-helix | 83-89 | 7 | |
| α-helix | 90-96 | 7 | |
| α-helix | 102-115 | 14 | |
| α-helix | 121-123 | 3 | |
| α-helix | 124-128 | 5 | |
| α-helix | 129-137 | 9 | |
| α-helix | 141-148 | 8 | |
| α-helix | 151-154 | 4 | |
| α-helix | 160-173 | 14 | |
| α-helix | 179-186 | 8 | |
| α-helix | 189-193 | 5 | |
| α-helix | 198-200 | 3 | |
| α-helix | 201-205 | 5 | |
| α-helix | 206-213 | 8 | |
| α-helix | 218-221 | 4 | |
| α-helix | 224-234 | 11 | |
| α-helix | 240-243 | 4 | |
| α-helix | 245-252 | 8 | |
| α-helix | 257-265 | 9 | |
| α-helix | 267-274 | 8 | |
| α-helix | 276-278 | 3 | |
| α-helix | 279-283 | 5 | |
| α-helix | 284-290 | 7 | |
| α-helix | 296-303 | 8 | |
| α-helix | 306-311 | 6 | |
| α-helix | 318-321 | 4 | |
| α-helix | 322-326 | 5 | |
| α-helix | 327-334 | 8 | |
| α-helix | 339-346 | 8 | |
| α-helix | 349-351 | 3 | |
| α-helix | 353-356 | 4 | |
| α-helix | 359-360 | 2 | |
| α-helix | 361-365 | 5 | |
| α-helix | 366-372 | 7 | |
| α-helix | 378-386 | 9 | |
| α-helix | 401-411 | 11 | |
| α-helix | 417-426 | 10 | |
| α-helix | 427-429 | 3 | |
| α-helix | 435-438 | 4 | |
| α-helix | 446-449 | 4 | |
| α-helix | 456-473 | 18 | |
| α-helix | 476-480 | 5 | |
| α-helix | 482-486 | 5 | |
| α-helix | 495-508 | 14 | |
| α-helix | 509-511 | 3 | |
| α-helix | 513-516 | 4 | |
| α-helix | 517-521 | 5 | |
| α-helix | 522-528 | 7 | |
| α-helix | 534-546 | 13 | |
| α-helix | 548-550 | 3 | |
| α-helix | 555 | 1 | |
| α-helix | 556-560 | 5 | |
| α-helix | 561-568 | 8 | |
| α-helix | 573-585 | 13 | |
Chain B: 63 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 26-33 | 8 | |
| α-helix | 34-36 | 3 | |
| α-helix | 38-41 | 4 | |
| α-helix | 44-46 | 3 | |
| α-helix | 47-51 | 5 | |
| α-helix | 52-54 | 3 | |
| α-helix | 63-73 | 11 | |
| α-helix | 77-80 | 4 | |
| α-helix | 83-89 | 7 | |
| α-helix | 90-96 | 7 | |
| α-helix | 102-116 | 15 | |
| α-helix | 121-123 | 3 | |
| α-helix | 124-128 | 5 | |
| α-helix | 129-137 | 9 | |
| α-helix | 141-148 | 8 | |
| α-helix | 151-154 | 4 | |
| α-helix | 160-174 | 15 | |
| α-helix | 179-193 | 15 | |
| α-helix | 198-200 | 3 | |
| α-helix | 201-205 | 5 | |
| α-helix | 206-212 | 7 | |
| α-helix | 218-221 | 4 | |
| α-helix | 224-234 | 11 | |
| α-helix | 237-243 | 7 | |
| α-helix | 245-252 | 8 | |
| α-helix | 257-265 | 9 | |
| α-helix | 267-274 | 8 | |
| α-helix | 276-278 | 3 | |
| α-helix | 279-283 | 5 | |
| α-helix | 284-290 | 7 | |
| α-helix | 296-303 | 8 | |
| α-helix | 306-312 | 7 | |
| α-helix | 319-321 | 3 | |
| α-helix | 322-326 | 5 | |
| α-helix | 327-333 | 7 | |
| α-helix | 339-346 | 8 | |
| α-helix | 349-352 | 4 | |
| α-helix | 354-356 | 3 | |
| α-helix | 357-360 | 4 | |
| α-helix | 361-365 | 5 | |
| α-helix | 366-372 | 7 | |
| α-helix | 378-385 | 8 | |
| α-helix | 389-392 | 4 | |
| α-helix | 405-410 | 6 | |
| α-helix | 417-431 | 15 | |
| α-helix | 435-438 | 4 | |
| α-helix | 446-449 | 4 | |
| α-helix | 456-473 | 18 | |
| α-helix | 475-477 | 3 | |
| α-helix | 478-482 | 5 | |
| α-helix | 483-488 | 6 | |
| α-helix | 489-491 | 3 | |
| α-helix | 495-511 | 17 | |
| α-helix | 515-516 | 2 | |
| α-helix | 517-521 | 5 | |
| α-helix | 522-527 | 6 | |
| α-helix | 528-530 | 3 | |
| α-helix | 534-547 | 14 | |
| α-helix | 548-550 | 3 | |
| α-helix | 553-555 | 3 | |
| α-helix | 556-560 | 5 | |
| α-helix | 561-568 | 8 | |
| α-helix | 573-585 | 13 | |
Chain C: 59 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-19 | 5 | |
| α-helix | 22-24 | 3 | |
| α-helix | 26-32 | 7 | |
| α-helix | 35-41 | 7 | |
| α-helix | 46-49 | 4 | |
| α-helix | 51-56 | 6 | |
| α-helix | 63-73 | 11 | |
| α-helix | 77-80 | 4 | |
| α-helix | 83-89 | 7 | |
| α-helix | 90-96 | 7 | |
| α-helix | 102-116 | 15 | |
| α-helix | 121-123 | 3 | |
| α-helix | 124-128 | 5 | |
| α-helix | 129-137 | 9 | |
| α-helix | 141-148 | 8 | |
| α-helix | 151-154 | 4 | |
| α-helix | 160-174 | 15 | |
| α-helix | 179-193 | 15 | |
| α-helix | 198-200 | 3 | |
| α-helix | 201-205 | 5 | |
| α-helix | 206-213 | 8 | |
| α-helix | 218-234 | 17 | |
| α-helix | 237-239 | 3 | |
| α-helix | 240-244 | 5 | |
| α-helix | 245-252 | 8 | |
| α-helix | 257-265 | 9 | |
| α-helix | 267-274 | 8 | |
| α-helix | 276-278 | 3 | |
| α-helix | 279-283 | 5 | |
| α-helix | 284-291 | 8 | |
| α-helix | 296-303 | 8 | |
| α-helix | 306-312 | 7 | |
| α-helix | 318-321 | 4 | |
| α-helix | 322-326 | 5 | |
| α-helix | 327-334 | 8 | |
| α-helix | 339-349 | 11 | |
| α-helix | 350-352 | 3 | |
| α-helix | 353-356 | 4 | |
| α-helix | 358-360 | 3 | |
| α-helix | 361-365 | 5 | |
| α-helix | 366-372 | 7 | |
| α-helix | 378-386 | 9 | |
| α-helix | 404-411 | 8 | |
| α-helix | 417-424 | 8 | |
| α-helix | 428-430 | 3 | |
| α-helix | 431-434 | 4 | |
| α-helix | 457-468 | 12 | |
| α-helix | 478-482 | 5 | |
| α-helix | 483-488 | 6 | |
| α-helix | 489-491 | 3 | |
| α-helix | 495-511 | 17 | |
| α-helix | 514-517 | 4 | |
| α-helix | 522-525 | 4 | |
| α-helix | 526-529 | 4 | |
| α-helix | 534-547 | 14 | |
| α-helix | 553-555 | 3 | |
| α-helix | 556-560 | 5 | |
| α-helix | 561-568 | 8 | |
| α-helix | 575-585 | 11 | |
Chain D: 53 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-18 | 5 | |
| α-helix | 29-33 | 5 | |
| α-helix | 35-38 | 4 | |
| α-helix | 46 | 1 | |
| α-helix | 47-51 | 5 | |
| α-helix | 52-54 | 3 | |
| α-helix | 65-74 | 10 | |
| α-helix | 83-89 | 7 | |
| α-helix | 90-96 | 7 | |
| α-helix | 102-116 | 15 | |
| α-helix | 121-123 | 3 | |
| α-helix | 124-128 | 5 | |
| α-helix | 129-136 | 8 | |
| α-helix | 141-148 | 8 | |
| α-helix | 151-154 | 4 | |
| α-helix | 155-157 | 3 | |
| α-helix | 160-174 | 15 | |
| α-helix | 179-186 | 8 | |
| α-helix | 189-193 | 5 | |
| α-helix | 198-200 | 3 | |
| α-helix | 201-205 | 5 | |
| α-helix | 206-212 | 7 | |
| α-helix | 218-232 | 15 | |
| α-helix | 240-243 | 4 | |
| α-helix | 245-251 | 7 | |
| α-helix | 257-264 | 8 | |
| α-helix | 267-278 | 12 | |
| α-helix | 279-283 | 5 | |
| α-helix | 284-290 | 7 | |
| α-helix | 296-303 | 8 | |
| α-helix | 306-312 | 7 | |
| α-helix | 318-321 | 4 | |
| α-helix | 322-326 | 5 | |
| α-helix | 327-334 | 8 | |
| α-helix | 339-349 | 11 | |
| α-helix | 350-356 | 7 | |
| α-helix | 357-360 | 4 | |
| α-helix | 361-365 | 5 | |
| α-helix | 366-373 | 8 | |
| α-helix | 378-386 | 9 | |
| α-helix | 389-392 | 4 | |
| α-helix | 401-411 | 11 | |
| α-helix | 417-426 | 10 | |
| α-helix | 429-432 | 4 | |
| α-helix | 444-448 | 5 | |
| α-helix | 456-477 | 22 | |
| α-helix | 478-482 | 5 | |
| α-helix | 483-486 | 4 | |
| α-helix | 496-508 | 13 | |
| α-helix | 538-540 | 3 | |
| α-helix | 541-544 | 4 | |
| α-helix | 555-558 | 4 | |
| α-helix | 577-584 | 8 | |
Chain E: 11 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-15 | 9 | |
| α-helix | 21-23 | 3 | |
| α-helix | 27-37 | 11 | |
| α-helix | 38-40 | 3 | |
| α-helix | 54-69 | 16 | |
| α-helix | 72 | 1 | |
| α-helix | 73-76 | 4 | |
| α-helix | 100-104 | 5 | |
| β-strand | 111 | 1 | 1 |
| α-helix | 114-125 | 12 | |
| β-strand | 135 | 1 | 2 |
| β-strand | 138 | 1 | 2 |
| α-helix | 141-147 | 7 | |
| α-helix | 154-164 | 11 | |
Chain F: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-17 | 11 | |
| α-helix | 27-37 | 11 | |
| α-helix | 52-69 | 18 | |
| α-helix | 100-105 | 6 | |
| α-helix | 114-125 | 12 | |
| α-helix | 141-147 | 7 | |
| α-helix | 154-164 | 11 | |
Chain G: 9 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-15 | 9 | |
| α-helix | 27-37 | 11 | |
| α-helix | 54-68 | 15 | |
| α-helix | 100-105 | 6 | |
| α-helix | 115-123 | 9 | |
| α-helix | 125-127 | 3 | |
| α-helix | 132-133 | 2 | |
| β-strand | 135 | 1 | 3 |
| β-strand | 138 | 1 | 3 |
| α-helix | 141-147 | 7 | |
| α-helix | 154-166 | 13 | |
Chain H: 7 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-15 | 9 | |
| α-helix | 27-33 | 7 | |
| α-helix | 54-63 | 10 | |
| β-strand | 69 | 1 | 4 |
| β-strand | 71 | 1 | 4 |
| α-helix | 101-104 | 4 | |
| β-strand | 111 | 1 | 1 |
| α-helix | 114-128 | 15 | |
| α-helix | 141-147 | 7 | |
| α-helix | 154-165 | 12 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform | A, B, C, D | protein | 589 | Mus musculus | Q76MZ3 (AlphaFold model) |
| Small T antigen | E, F, G, H | protein | 174 | Simian virus 40 | P03081 (AlphaFold model) |
Sequence of entity 1 (A, B, C, D), FASTA
>2PF4_1 Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (chains A, B, C, D)
MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIY
DEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHS
PSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPM
VRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDL
EALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRA
AASHKVKEFCENLSADCRENVIMTQILPCIKELVSDANQHVKSALASVIMGLSPILGKDN
TIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVR
LAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHA
TIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNV
AKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
Sequence of entity 2 (E, F, G, H), FASTA
>2PF4_2 Small T antigen (chains E, F, G, H)
MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYK
KMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAIYCKQWPECVKKMSTNCICLLCLLRM
KHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDIIGQTTYRDLKL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 8 |
Primary citation
Structural basis of PP2A inhibition by small t antigen. Cho, U.S., Morrone, S., Sablina, A.A. et al. PLoS Biol (2007) 5:e202-e202. DOI 10.1371/journal.pbio.0050202 · PubMed
Other PDB entries of the same protein (UniProt Q76MZ3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 3FGA 2.7 Å, Structural Basis of PP2A and Sgo interaction
- 6EF4 3.4 Å, Crystal structure of mouse PP2A Aalpha P179R mutant
- 2IAE 3.5 Å, Crystal structure of a protein phosphatase 2A (PP2A) holoenzyme.
Browse structure collections
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