2WUW: Subtilisin carlsberg

Crystallographic analysis of counter-ion effects on subtilisin enzymatic action in acetonitrile (native data). Determined by X-ray diffraction at 2.23 Å resolution. Released 8 Dec 2010.

Method: X-ray diffraction
Resolution: 2.23 Å
Organism: BACILLUS LICHENIFORMIS
Chains: 1
Atoms: 2,068
Mol. weight: 27.69 kDa
Ligands: CA, CCN
Released: 8 Dec 2010

Explore 2WUW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2WUW contains 11 α-helices and 14 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain E: 11 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	13-18	6
β-strand	27-32	6	1
β-strand	44-49	6	1
α-helix	64-73	10
β-strand	89-94	6	1
α-helix	104-116	13
β-strand	121-124	4	1
β-strand	128	1	2
α-helix	133-144	12
β-strand	148-152	5	1
β-strand	167	1	2
β-strand	175-180	6	1
α-helix	185	1
β-strand	186	1	1
α-helix	187	1
β-strand	196-201	6	1
β-strand	205-209	5	3
β-strand	213-217	5	3
α-helix	220-237	18
α-helix	243-252	10
β-strand	255	1	1
α-helix	260-263	4
β-strand	267	1	1
α-helix	270-273	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Subtilisin carlsberg	E	protein	274	BACILLUS LICHENIFORMIS	P00780 (AlphaFold model)

Sequence of entity 1 (E), FASTA

>2WUW_1 SUBTILISIN CARLSBERG (chains E)
AQTVPYGIPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYNTDG
NGHGTHVAGTVAALDNTTGVLGVAPSVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDV
INMSLGGASGSTAMKQAVDNAYARGVVVVAAAGNSGNSGSTNTIGYPAKYDSVIAVGAVD
SNSNRASFSSVGAELEVMAPGAGVYSTYPTNTYATLNGTSMASPHVAGAAALILSKHPNL
SASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1
CCN	Acetonitrile	C2 H3 N	5

Water and common crystallization additives (SO4, NA) are not listed.

Primary citation

Crystallographic Analysis of Counterion Effects on Subtilisin Enzymatic Action in Acetonitrile. Cianci, M., Tomaszewski, B., Helliwell, J.R. et al. J Am Chem Soc (2010) 132:2293. DOI 10.1021/JA908703C · PubMed

Other PDB entries of the same protein (UniProt P00780 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1R0R 1.1 Å, 1.1 Angstrom Resolution Structure of the Complex Between the Protein Inhibitor, OMTKY3,…
1CSE 1.2 Å, The high-resolution X-ray crystal structure of the complex formed between subtilisin…
3UNX 1.26 Å, Bond length analysis of asp, glu and his residues in subtilisin Carlsberg at 1.26A…
6DWQ 1.27 Å, Subtilisin serine protease modified with the protease inhibitor…
1YU6 1.55 Å, Crystal Structure of the Subtilisin Carlsberg:OMTKY3 Complex
2SEC 1.8 Å, Structural comparison of two serine proteinase-protein inhibitor complexes.…
1SCN 1.9 Å, Inactivation of subtilisin carlsberg by N-(tert-butoxycarbonyl-alanyl-prolyl-phenylalanyl…
1AVT 2.0 Å, Subtilisin carlsberg D-para-chlorophenyl-1-acetamido boronic acid inhibitor complex
1SCA 2.0 Å, Enzyme crystal structure in a neat organic solvent
1SEL 2.0 Å, Crystal structure of selenosubtilisin at 2.0-Å resolution
1VSB 2.1 Å, Subtilisin carlsberg L-para-chlorophenyl-1-acetamido boronic acid inhibitor complex
1BE6 2.15 Å, Trans-cinnamoyl-subtilisin in anhydrous acetonitrile

Browse structure collections

About this viewer

MolViewer shows 2WUW directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.