2XWU: Importin 13 - UBC9 complex

Crystal structure of importin 13 - UBC9 complex. Determined by X-ray diffraction at 2.8 Å resolution. Released 29 Dec 2010.

Method: X-ray diffraction
Resolution: 2.8 Å
Organism: HOMO SAPIENS
Chains: 2
Atoms: 8,134
Mol. weight: 126.32 kDa
Released: 29 Dec 2010

Explore 2XWU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2XWU contains 68 α-helices and 9 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	2-18	17
β-strand	25-30	6	1
β-strand	36-46	11	1
α-helix	47-48	2
β-strand	57-63	7	1
α-helix	72-73	2
β-strand	74-77	4	1
β-strand	86	1	2
β-strand	91	1	1
β-strand	92	1	2
α-helix	95-97	3
α-helix	109-121	13
α-helix	131-139	9
α-helix	141-154	14

Chain B: 61 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	23-35	13
α-helix	39-54	16
α-helix	58-64	7
α-helix	72-88	17
α-helix	90-92	3
α-helix	95-97	3
α-helix	98-111	14
α-helix	117-134	18
α-helix	142-151	10
α-helix	159-179	21
α-helix	184-210	27
α-helix	216-230	15
α-helix	236-249	14
α-helix	256-267	12
α-helix	270-274	5
α-helix	276-287	12
α-helix	290-299	10
α-helix	302-318	17
α-helix	320-325	6
α-helix	327-329	3
α-helix	330-344	15
α-helix	357-360	4
α-helix	361-373	13
α-helix	377-400	24
α-helix	403-405	3
α-helix	406-410	5
α-helix	414-438	25
α-helix	440-455	16
α-helix	463-477	15
α-helix	487-494	8
α-helix	495-497	3
α-helix	503-515	13
α-helix	517-522	6
α-helix	524-526	3
α-helix	527-537	11
α-helix	541-543	3
α-helix	544-557	14
α-helix	559-561	3
α-helix	566-578	13
α-helix	584-599	16
α-helix	603-625	23
α-helix	631-650	20
α-helix	676-695	20
α-helix	699-716	18
α-helix	717-723	7
α-helix	724-737	14
α-helix	741-754	14
α-helix	762-781	20
α-helix	783-785	3
α-helix	787-803	17
α-helix	805-809	5
α-helix	815-825	11
α-helix	831-844	14
α-helix	845-847	3
β-strand	848	1	3
β-strand	851	1	3
α-helix	852-871	20
α-helix	872-876	5
α-helix	879-881	3
α-helix	882-895	14
α-helix	897-908	12
α-helix	920-930	11
α-helix	937-952	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Sumo-conjugating enzyme UBC9	A	protein	158	HOMO SAPIENS	P63279 (AlphaFold model)
IMPORTIN13	B	protein	963	HOMO SAPIENS	O94829 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2XWU_1 SUMO-CONJUGATING ENZYME UBC9 (chains A)
MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKL
RMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELL
NEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Sequence of entity 2 (B), FASTA

>2XWU_2 IMPORTIN13 (chains B)
MERREEQPGAAGAGAAPALDFTVENVEKALHQLYYDPNIENKNLAQKWLMQAQVSPQAWH
FSWQLLQPDKVPEIQYFGASALHIKISRYWSDIPTDQYESLKAQLFTQITRFASGSKIVL
TRLCVALASLALSMMPDAWPCAVADMVRLFQAEDSPVDGQGRCLALLELLTVLPEEFQTS
RLPQYRKGLVRTSLAVECGAVFPLLEQLLQQPSSPSCVRQKVLKCFSSWVQLEVPLQDCE
ALIQAAFAALQDSELFDSSVEAIVNAISQPDAQRYVNTLLKLIPLVLGLQEQLRQAVQNG
DMETSHGICRIAVALGENHSRALLDQVEHWQSFLALVNMIMFCTGIPGHYPVNETTSSLT
LTFWYTLQDDILSFEAEKQAVYQQVYRPVYFQLVDVLLHKAQFPSDEEYGFWSSDEKEQF
RIYRVDISDTLMYVYEMLGAELLSNLYDKLGRLLTSSEEPYSWQHTEALLYGFQSIAETI
DVNYSDVVPGLIGLIPRISISNVQLADTVMFTIGALSEWLADHPVMINSVLPLVLHALGN
PELSVSSVSTLKKICRECKYDLPPYAANIVAVSQDVLMKQIHKTSQCMWLMQALGFLLSA
LQVEEILKNLHSLISPYIQQLEKLAEEIPNPSNKLAIVHILGLLSNLFTTLDISHHEDDH
EGPELRKLPVPQGPNPVVVVLQQVFQLIQKVLSKWLNDAQVVEAVCAIFEKSVKTLLDDF
APMVPQLCEMLGRMYSTIPQASALDLTRQLVHIFAHEPAHFPPIEALFLLVTSVTLTLFQ
QGPRDHPDIVDSFMQLLAQALKRKPDLFLCERLDVKAVFQCAVLALKFPEAPTVKASCGF
FTELLPRCGEVESVGKVVQEDGRMLLIAVLEAIGGQASRSLMDCFADILFALNKHCFSLL
SMWIKEALQPPGFPSARLSPEQKDTFSQQILRERVNKRRVKEMVKEFTLLCRGLHGTDYT
ADY

Primary citation

Structure of Importin13-Ubc9 Complex: Nuclear Import and Release of a Key Regulator of Sumoylation. Gruenwald, M., Bono, F. EMBO J (2011) 30:427. DOI 10.1038/EMBOJ.2010.320 · PubMed

Other PDB entries of the same protein (UniProt P63279 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5F6E 1.12 Å, Crystal Structure of human Ubc9 (K48A/K49A/E54A)
5F6Y 1.14 Å, Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 2 (mercaptobenzoxazole)
2GRR 1.3 Å, Crystal Structure of human RanGAP1-Ubc9-D127S
5F6V 1.49 Å, Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 1 (biphenol from…
5F6U 1.55 Å, Crystal Structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 8 (JSS190B146)
5F6D 1.55 Å, Crystal structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 6
5F6X 1.56 Å, Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 2 (mercaptobenzoxazole…
5F6W 1.7 Å, Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 1 (biphenol)
2GRO 1.7 Å, Crystal Structure of human RanGAP1-Ubc9-N85Q
2GRQ 1.7 Å, Crystal Structure of human RanGAP1-Ubc9-D127A
9GLR 1.72 Å, Crystal Structure of Human UBC9 C93E
2GRN 1.8 Å, Crystal Structure of human RanGAP1-Ubc9

Browse structure collections

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