3AJI: 26S proteasome non-ATPase regulatory subunit 10

Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion. Determined by X-ray diffraction at 2.05 Å resolution. Released 22 Dec 2010.

Method: X-ray diffraction
Resolution: 2.05 Å
Organism: Mus musculus
Chains: 4
Atoms: 4,995
Mol. weight: 69.95 kDa
Released: 22 Dec 2010

Explore 3AJI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3AJI contains 39 α-helices and 4 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	9-16	8
α-helix	19-28	10
α-helix	30-34	5
α-helix	43-50	8
α-helix	53-61	9
α-helix	76-83	8
α-helix	86-94	9
α-helix	109-115	7
α-helix	119-127	9
α-helix	142-149	8
α-helix	152-160	9
α-helix	175-181	7
α-helix	185-193	9
α-helix	208-211	4
α-helix	214-229	16

Chain B: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	338-349	12
β-strand	354	1	1
α-helix	361-364	4
α-helix	372-384	13
α-helix	385-387	3
β-strand	394	1	1
α-helix	396-406	11

Chain C: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	9-15	7
α-helix	19-28	10
α-helix	30-34	5
α-helix	43-50	8
α-helix	53-62	10
α-helix	76-83	8
α-helix	86-94	9
α-helix	109-115	7
α-helix	119-127	9
α-helix	142-149	8
α-helix	152-160	9
α-helix	175-181	7
α-helix	185-193	9
α-helix	208-211	4
α-helix	214-229	16

Chain D: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	338-349	12
β-strand	354	1	2
α-helix	361-364	4
α-helix	372-387	16
β-strand	394	1	2
α-helix	396-406	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
26S proteasome non-ATPase regulatory subunit 10	A, C	protein	231	Mus musculus	Q9Z2X2 (AlphaFold model)
Proteasome (Prosome, macropain) 26S subunit, ATPase, 4	B, D	protein	83	Mus musculus	P54775 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>3AJI_1 26S proteasome non-ATPase regulatory subunit 10 (chains A, C)
MEGCVSNIMICNLAYSGKLDELKERILADKSLATRTDQDSRTALHWACSAGHTEIVEFLL
QLGVPVNDKDDAGWSPLHIAASAGFDEIVKALLVKGAHVNAVNQNGCTPLHYAASKNRHE
IAVMLLEGGANPDAKDHYDATAMHRAAAKGNLKMVHILLFYKASTNIQDTEGNTPLHLAC
DEERVEEAKFLVTQGASIYIENKEEKTPLQVAKGGLGLILKRLAEGEEASM

Sequence of entity 2 (B, D), FASTA

>3AJI_2 Proteasome (Prosome, macropain) 26S subunit, ATPase, 4 (chains B, D)
MDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVL
AKDFEKAYKTVIKKDEQEHEFYK

Primary citation

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid. Sato, S., Mimasu, S., Sato, A. et al. Biochemistry (2011) 50:250-257. DOI 10.1021/bi1016183 · PubMed

Other PDB entries of the same protein (UniProt Q9Z2X2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2DVW 2.3 Å, Structure of the Oncoprotein Gankyrin in Complex with S6 ATPase of the 26S Proteasome
2DWZ 2.4 Å, Structure of the Oncoprotein Gankyrin in Complex with S6 ATPase of the 26S Proteasome

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