3BIP: Yeast Spt16 N-terminal Domain

Crystal structure of yeast Spt16 N-terminal Domain. Determined by X-ray diffraction at 1.94 Å resolution. Released 18 Dec 2007.

Method: X-ray diffraction
Resolution: 1.94 Å
Organism: Saccharomyces cerevisiae
Chains: 2
Atoms: 8,034
Mol. weight: 106.83 kDa
Released: 18 Dec 2007

Explore 3BIP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3BIP contains 41 α-helices and 42 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	8-21	14
α-helix	22-24	3
β-strand	31-36	6	1
α-helix	47-56	10
β-strand	63-68	6	1
β-strand	71-76	6	1
α-helix	78-90	13
β-strand	99-104	6	1
α-helix	110-127	18
β-strand	130-133	4	1
α-helix	142-158	17
β-strand	161-164	4	1
α-helix	166-173	8
α-helix	178-207	30
β-strand	213	1	2
α-helix	214-222	9
α-helix	223-226	4
α-helix	228-239	12
α-helix	243	1
α-helix	250-252	3
β-strand	253-255	3	3
β-strand	260-262	3	4
β-strand	279	1	2
α-helix	280	1
β-strand	284-290	7	4
β-strand	292-294	3	3
β-strand	297	1	3
β-strand	301-307	7	4
α-helix	311-326	16
α-helix	327-331	5
α-helix	338-352	15
α-helix	354-359	6
β-strand	360	1	4
β-strand	365-367	3	4
α-helix	375-377	3
β-strand	378	1	4
β-strand	393-403	11	4
β-strand	412-421	10	4
α-helix	422-423	2
α-helix	428-430	3
β-strand	431-432	2	4
α-helix	440-442	3
β-strand	444-445	2	4

Chain B: 19 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	8-21	14
α-helix	22-24	3
β-strand	31-36	6	5
α-helix	47-56	10
β-strand	63-68	6	5
β-strand	71-77	7	5
α-helix	78-88	11
α-helix	89-91	3
β-strand	99-105	7	5
α-helix	110-127	18
β-strand	130-133	4	5
α-helix	142-158	17
β-strand	161-164	4	5
α-helix	166-172	7
α-helix	178-207	30
β-strand	213	1	6
α-helix	214-223	10
α-helix	228-239	12
α-helix	243	1
α-helix	250-252	3
β-strand	253-255	3	7
β-strand	260-262	3	8
β-strand	279	1	6
β-strand	284-290	7	8
β-strand	292-294	3	7
β-strand	297-298	2	7
β-strand	301-307	7	8
α-helix	311-326	16
α-helix	327-331	5
α-helix	338-352	15
α-helix	354-359	6
β-strand	360	1	8
β-strand	365-367	3	8
β-strand	378	1	8
β-strand	393-405	13	8
β-strand	408-421	14	8
α-helix	429-430	2
β-strand	431-432	2	8
α-helix	440-443	4
β-strand	444-445	2	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
FACT complex subunit SPT16	A, B	protein	467	Saccharomyces cerevisiae	P32558 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3BIP_1 FACT complex subunit SPT16 (chains A, B)
GHMEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENPYQKTTILHNWLLSY
EFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLELWQRNNKEPELNKKLFD
DVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEFNVIDISLGLSKVWEVKDV
NEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLSDKIENKIDDVKFLKQLSPDL
SALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARSTNDQLYGNGCILASCGIRYNNYC
SNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKPGRTPKEVYESVIEYIEKTKPELVP
NFTKNIGSLIGLEFRDSNFILNVKNDYRKIQRGDCFNISFGFNNLKDSQSANNYALQLAD
TVQIPLDETEPPRFLTNYTKAKSQISFYFNNEEEDNNKKKSSPATKV

Primary citation

Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits. VanDemark, A.P., Xin, H., McCullough, L. et al. J Biol Chem (2008) 283:5058-5068. DOI 10.1074/jbc.M708682200 · PubMed

Other PDB entries of the same protein (UniProt P32558 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3BIQ 1.73 Å, Crystal structure of yeast Spt16 N-terminal Domain
4WNN 1.8 Å, SPT16-H2A-H2B FACT HISTONE Complex
3BIT 1.9 Å, Crystal structure of yeast Spt16 N-terminal Domain
4IOY 1.95 Å, Structure of the Spt16 Middle Domain Reveals Functional Features of the Histone…
7NKY 3.2 Å, RNA Polymerase II-Spt4/5-nucleosome-FACT structure
8XGC 3.7 Å, Structure of yeast replisome associated with FACT and histone hexamer, Composite map

Browse structure collections

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