3CFC: Blue fluorescent antibody EP2-19G2

High-resolution structure of blue fluorescent antibody EP2-19G2. Determined by X-ray diffraction at 1.7 Å resolution. Released 18 Mar 2008.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Mus musculus
Chains: 2
Atoms: 3,703
Mol. weight: 46.92 kDa
Released: 18 Mar 2008

Explore 3CFC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3CFC contains 17 α-helices and 45 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain H: 7 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	10-12	3	2
β-strand	18-25	8	1
α-helix	29-31	3
β-strand	34-39	6	2
β-strand	46-51	6	2
β-strand	57-58	2	2
β-strand	68-72	5	1
β-strand	77-82	6	1
α-helix	84-86	3
β-strand	88-94	7	2
β-strand	102-103	2	2
β-strand	107-111	5	2
β-strand	117	1	3
α-helix	118-119	2
β-strand	120-124	5	4
α-helix	126-127	2
β-strand	137-147	11	4
β-strand	148	1	3
β-strand	153-157	4	5
α-helix	162-164	3
α-helix	167-169	3
β-strand	171-179	9	4
β-strand	184-194	11	4
β-strand	206-212	6	5
α-helix	213-215	3
β-strand	217-222	6	5

Chain L: 10 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	6
β-strand	9	1	7
α-helix	10-12	3
β-strand	13-14	2	8
β-strand	19-25	7	6
β-strand	27C	1	9
β-strand	31	1	9
β-strand	33-38	6	7
β-strand	45-49	5	7
β-strand	53-54	2	7
α-helix	55	1
β-strand	62-67	6	6
β-strand	70-75	6	6
α-helix	80-82	3
β-strand	84-90	7	7
α-helix	96	1
β-strand	97-98	2	7
β-strand	102-104	3	7
β-strand	106-107	2	8
β-strand	111	1	10
α-helix	112-113	2
β-strand	114-118	5	11
α-helix	119-121	3
α-helix	122-126	5
β-strand	129-139	11	11
β-strand	140	1	10
β-strand	145-150	6	12
β-strand	153-155	3	12
β-strand	159-163	5	11
α-helix	164-167	4
β-strand	173-182	10	11
α-helix	183-186	4
β-strand	191-197	7	12
α-helix	204	1
β-strand	205-210	6	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Blue fluorescent antibody EP2-19G2-IGG2B heavy chain	H	protein	213	Mus musculus
Blue fluorescent antibody EP2-19G2-kappa light chain	L	protein	219	Mus musculus	P01837 (AlphaFold model), P03976 (AlphaFold model)

Sequence of entity 1 (H), FASTA

>3CFC_1 BLUE FLUORESCENT ANTIBODY EP2-19G2-IGG2B HEAVY CHAIN (chains H)
EVKLVESGGGLVKPGGSLKLSCTASGITFSRYIMSWVRQIPEKRLEWVASISSGGITYYP
DSVKGRFTISRDNVRNILYLQMSSLRSEDTALYYCARGQGRPYWGQGTLVTVSSAKTTPP
SVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSS
SVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP

Sequence of entity 2 (L), FASTA

>3CFC_2 BLUE FLUORESCENT ANTIBODY EP2-19G2-KAPPA LIGHT CHAIN (chains L)
DIVMTQAAFSNPVTLGTSASISCRSTKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLA
SGVPDRFSSSGSGTDFTLRISRVEAEDVGVYYCAQNLELPPTFGGGTKLEIKRADAAPTV
SIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSM
SSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Primary citation

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex. Debler, E.W., Kaufmann, G.F., Meijler, M.M. et al. Science (2008) 319:1232-1235. DOI 10.1126/science.1153445 · PubMed

Other PDB entries of the same protein (UniProt P01837 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3D9A 1.2 Å, High Resolution Crystal Structure Structure of HyHel10 Fab Complexed to Hen Egg Lysozyme
1RUR 1.5 Å, Crystal Structure (I) of native Diels-Alder antibody 13G5 Fab at pH 8.0 with a data set…
2AJU 1.5 Å, Cyrstal structure of cocaine catalytic antibody 7A1 Fab'
2AJV 1.5 Å, Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with Cocaine
2W60 1.5 Å, Anti citrullinated Collagen type 2 antibody acc4
2W9D 1.57 Å, Structure of Fab fragment of the ICSM 18 - anti-Prp therapeutic antibody at 1.57 A…
3BKJ 1.59 Å, Crystal structure of Fab wo2 bound to the n terminal domain of amyloid beta peptide (1-16)
3BAE 1.59 Å, Crystal structure of Fab WO2 bound to the N terminal domain of Amyloid beta peptide (1-28)
2R1Y 1.6 Å, Crystal structure of S25-2 Fab in complex with Kdo analogues
2R2B 1.6 Å, Crystal structure of S25-2 Fab in complex with Kdo analogues
3BKM 1.6 Å, Structure of anti-amyloid-beta Fab WO2 (Form A, P212121)
3CFB 1.6 Å, High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene…

Browse structure collections

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