3E4Z: Human insulin degrading enzyme

Crystal structure of human insulin degrading enzyme in complex with insulin-like growth factor II. Determined by X-ray diffraction at 2.28 Å resolution. Released 18 Aug 2009.

Method

X-ray diffraction

Resolution

2.28 Å

Organism

Homo sapiens

Chains

4

Atoms

16,411

Mol. weight

244.22 kDa

Ligands

ZN

Released

18 Aug 2009

Explore 3E4Z in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3E4Z contains 121 α-helices and 72 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 60 helices, 34 β-strands

Molecules and chains

>3E4Z_1 Insulin-degrading enzyme (chains A, B)
MHHHHHHAAGIPMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAA
LDVHIGSLSDPPNIAGLSHFLQHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNY
YFDVSHEHLEGALDRFAQFFLSPLFDESAKDREVNAVDSEHEKNVMNDAWRLFQLEKATG
NPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVVVLGRESLDDLTN
LVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSN
PGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVED
IILHMFQYIQKLRAEGPQEWVFQELKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEV
LTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEV
IKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFL
PKANLNFEFFSPFAYVDPLHSNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYL
SVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLL
MTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTL
IEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNSGIEIYYQTDMQSTSENMFLE
LFAQIISEPAFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLIT
MEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAESAKYYGEIISQQYNFDRDNTEVAYLK
TLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQP
EVIQNMTEFKRGLPLFPLVKPHINFMAAKL

>3E4Z_2 Insulin-like growth factor II (chains C, D)
AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYC
ATPAKSE

Ligands and cofactors

Primary citation

Molecular Basis for the Recognition and Cleavages of IGF-II, TGF-alpha, and Amylin by Human Insulin-Degrading Enzyme. Guo, Q., Manolopoulou, M., Bian, Y. et al. J Mol Biol (2010) 395:430-443. DOI 10.1016/j.jmb.2009.10.072 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P14735 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3CWW 1.96 Å, Crystal Structure of IDE-bradykinin complex
• 2G47 2.1 Å, Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40)
• 2G56 2.2 Å, crystal structure of human insulin-degrading enzyme in complex with insulin B chain
• 4PES 2.21 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl…
• 4PFC 2.21 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 2G54 2.25 Å, Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B…
• 3E50 2.3 Å, Crystal structure of human insulin degrading enzyme in complex with transforming growth…
• 4PF7 2.33 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 3OFI 2.35 Å, Crystal structure of human insulin-degrading enzyme in complex with ubiquitin
• 2G49 2.5 Å, Crystal structure of human insulin-degrading enzyme in complex with glucagon
• 4PF9 2.5 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 2WK3 2.59 Å, Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42)

Browse structure collections

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