4PFC: Insulin degrading enzyme

Crystal structure of insulin degrading enzyme complexed with inhibitor. Determined by X-ray diffraction at 2.21 Å resolution. Released 17 Jun 2015.

Method

X-ray diffraction

Resolution

2.21 Å

Organism

Homo sapiens

Chains

2

Atoms

16,390

Mol. weight

230.59 kDa

Ligands

2QX, ZN

Released

17 Jun 2015

Explore 4PFC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4PFC contains 112 α-helices and 68 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 54 helices, 34 β-strands

Molecules and chains

>4PFC_1 Insulin-degrading enzyme (chains A, B)
MGHHHHHHGRAMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAAL
DVHIGSLSDPPNIAGLSHFLQHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYY
FDVSHEHLEGALDRFAQFFLSPLFDESAKDREVNAVDSEHEKNVMNDAWRLFQLEKATGN
PKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVVVLGRESLDDLTNL
VVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNP
GHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDI
ILHMFQYIQKLRAEGPQEWVFQELKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVL
TAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVI
KKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLP
KANLNFEFFSPFAYVDPLHSNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLS
VKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLM
TEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLI
EHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNSGIEIYYQTDMQSTSENMFLEL
FAQIISEPAFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITM
EKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAESAKYWGEIISQQYNFDRDNTEVAYLKT
LTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSNPVVGEFPAQNDINLSQAPALPQPE
VIQNMTEFKRGLPLFPLVKPHINFMAAKL

Ligands and cofactors

Primary citation

Dual Exosite-binding Inhibitors of Insulin-degrading Enzyme Challenge Its Role as the Primary Mediator of Insulin Clearance in Vivo. Durham, T.B., Toth, J.L., Klimkowski, V.J. et al. J Biol Chem (2015) 290:20044-20059. DOI 10.1074/jbc.M115.638205 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P14735 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3CWW 1.96 Å, Crystal Structure of IDE-bradykinin complex
• 2G47 2.1 Å, Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40)
• 2G56 2.2 Å, crystal structure of human insulin-degrading enzyme in complex with insulin B chain
• 4PES 2.21 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl…
• 2G54 2.25 Å, Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B…
• 3E4Z 2.28 Å, Crystal structure of human insulin degrading enzyme in complex with insulin-like growth…
• 3E50 2.3 Å, Crystal structure of human insulin degrading enzyme in complex with transforming growth…
• 4PF7 2.33 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 3OFI 2.35 Å, Crystal structure of human insulin-degrading enzyme in complex with ubiquitin
• 2G49 2.5 Å, Crystal structure of human insulin-degrading enzyme in complex with glucagon
• 4PF9 2.5 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 2WK3 2.59 Å, Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42)

Browse structure collections

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