3EZA: Phosphotransferase system, enzyme I

Complex of the amino terminal domain of enzyme I and the histidine-containing phosphocarrier protein hpr from escherichia coli NMR, restrained regularized mean structure. Determined by solution NMR. Released 25 May 1999.

Method: Solution NMR
Organism: Escherichia coli
Chains: 2
Atoms: 2,547
Mol. weight: 36.35 kDa
Released: 25 May 1999

Explore 3EZA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3EZA contains 14 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	8	1	1
β-strand	12-18	7	1
α-helix	22-25	4
α-helix	29-32	4
α-helix	33-64	32
α-helix	67-80	14
α-helix	83-91	9
α-helix	92-96	5
α-helix	100-116	17
α-helix	121-142	22
β-strand	156-160	5	1
α-helix	165-169	5
β-strand	176-181	6	1
α-helix	189-197	9
β-strand	201-202	2	1
β-strand	216-220	5	1
β-strand	227-229	3	1
α-helix	233-248	16

Chain B: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	2
α-helix	16-26	11
β-strand	32-37	6	2
β-strand	40-43	4	2
α-helix	47-50	4
β-strand	60-66	7	2
α-helix	70-83	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Phosphotransferase system, enzyme I	A	protein	249	Escherichia coli	P08839 (AlphaFold model)
Histidine-containing phosphocarrier protein hpr	B	protein	85	Escherichia coli	P0AA04 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3EZA_1 PHOSPHOTRANSFERASE SYSTEM, ENZYME I (chains A)
MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFLSGRAKASAQLETIKTK
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQASALEELDD
EYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVAADLTPSETAQLNLKKVLGFI
TDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYLILDAVNNQVYVNPTNEVIDKMR
AVQEQVASE

Sequence of entity 2 (B), FASTA

>3EZA_2 HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR (chains B)
MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV
VTISAEGEDEQKAVEHLVKLMAELE

Primary citation

Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr. Garrett, D.S., Seok, Y.J., Peterkofsky, A. et al. Nat Struct Biol (1999) 6:166-173. DOI 10.1038/5854 · PubMed

Other PDB entries of the same protein (UniProt P08839 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6V9K 1.9 Å, Crystal structure of the hybrid C-terminal domain of enzyme I of the bacterial…
1ZYM 2.5 Å, Amino terminal domain of enzyme I from escherichia coli
2HWG 2.7 Å, Structure of phosphorylated Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase…
6VU0 3.5 Å, Crystal structure of the C-terminal domain of enzyme I of the bacterial…
1EZA Amino terminal domain of enzyme I from escherichia coli NMR, restrained regularized mean…
1EZB Amino terminal domain of enzyme I from escherichia coli, NMR, 17 structures
1EZC Amino terminal domain of enzyme I from escherichia coli, NMR, 17 structures
1EZD Amino terminal domain of enzyme I from escherichia coli NMR, 16 structures
2EZA Amino terminal domain of enzyme I from escherichia coli, NMR, restrained regularized…
2EZB Amino terminal domain of enzyme I from escherichia coli, NMR, 14 structures
2EZC Amino terminal domain of enzyme I from escherichia coli, NMR, 14 structures
2KX9 Solution Structure of the Enzyme I dimer Using Residual Dipolar Couplings and Small…

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