3GLI: E. coli clamp loader

Crystal Structure of the E. coli clamp loader bound to Primer-Template DNA and Psi Peptide. Determined by X-ray diffraction at 3.5 Å resolution. Released 26 May 2009.

Method

X-ray diffraction

Resolution

3.5 Å

Organisms

Escherichia coli, Escherichia coLI, ESCHERICHIA COLI

Chains

16

Atoms

29,139

Mol. weight

440.4 kDa

Ligands

ADP, BEF, ZN, MG

Released

26 May 2009

Explore 3GLI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3GLI contains 231 α-helices and 83 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 8 β-strands

3 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

>3GLI_1 DNA polymerase III subunit delta (chains A, F)
MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTD
WNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKA
QENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLA
LAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEG
SEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQ
AVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG

>3GLI_2 DNA polymerase III subunit tau (chains B, C, D, G, H, I)
MGSSHHHHHHSSGLEVLFQGPHMSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIH
HAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASR
TKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTD
PQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEW
EALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKE
LPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQ

>3GLI_3 DNA polymerase III subunit delta' (chains E, J)
MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCG
HCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALL
TDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREV
TMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQ
APARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQL
MSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL

>3GLI_4 DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3') (chains K, M)
TTTTTTATAGGCCAG

>3GLI_5 DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3') (chains L, N)
CTGGCCTATA

>3GLI_6 DNA polymerase III subunit psi (chains O, P)
TSRRDWQLQQLGITQWSLRRPGALQGE

Ligands and cofactors

Primary citation

The mechanism of ATP-dependent primer-template recognition by a clamp loader complex. Simonetta, K.R., Kazmirski, S.L., Goedken, E.R. et al. Cell (2009) 137:659-671. DOI 10.1016/j.cell.2009.03.044 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P28630 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1JQL 2.5 Å, Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader…
• 9OYJ 2.53 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 9OYH 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 9OYI 2.54 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 8GJ2 2.6 Å, E. coli clamp loader with closed clamp on primed template DNA
• 9OYK 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 9OYM 2.6 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 1JR3 2.7 Å, Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA…
• 8GIZ 2.7 Å, E. coli clamp loader with open clamp
• 9OYN 2.7 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped…
• 9OYC 2.71 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…
• 9OYE 2.72 Å, Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 1-nt gapped…

Browse structure collections

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