3HFM: Hyhel-10 IgG1 FAB

Structure of an antibody-antigen complex. Crystal structure of the hy/hel-10 FAB-lysozyme complex. Determined by X-ray diffraction at 3.0 Å resolution. Released 12 Jul 1989.

Method: X-ray diffraction
Resolution: 3.0 Å
Organisms: Mus musculus, Gallus gallus
Chains: 3
Atoms: 4,297
Mol. weight: 61.21 kDa
Released: 12 Jul 1989

Explore 3HFM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3HFM contains 16 α-helices and 55 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain H: 2 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	6
β-strand	11-12	2	7
β-strand	18-25	8	6
β-strand	34-39	6	8
β-strand	45-52	8	8
β-strand	56-59	4	8
β-strand	70-72	3	6
β-strand	77-82	6	6
α-helix	87-89	3
β-strand	91-97	7	8
β-strand	107-109	3	8
β-strand	110-111	2	7
β-strand	117	1	9
β-strand	120-124	5	10
β-strand	135-145	11	10
β-strand	146	1	9
β-strand	153-154	2	11
β-strand	159	1	11
β-strand	163-165	3	10
α-helix	166-168	3
β-strand	169-170	2	10
β-strand	175-184	10	10
β-strand	193-196	4	11
β-strand	199	1	12
β-strand	204	1	12
β-strand	207-210	4	11

Chain L: 6 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	1
β-strand	10-13	4	2
β-strand	21-25	5	1
β-strand	33-38	6	2
β-strand	45-49	5	2
β-strand	53-54	2	2
α-helix	55	1
β-strand	62-66	5	1
β-strand	70-75	6	1
α-helix	80-82	3
β-strand	84-90	7	2
α-helix	96	1
β-strand	98	1	2
β-strand	103-106	4	2
β-strand	111	1	3
β-strand	114-118	5	4
α-helix	122-127	6
β-strand	131-137	7	4
β-strand	140	1	3
β-strand	145-150	6	5
β-strand	154	1	5
β-strand	159-163	5	4
α-helix	165-167	3
β-strand	174-180	7	4
α-helix	183-185	3
β-strand	191-197	7	5
β-strand	205-210	6	5

Chain Y: 8 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	13
α-helix	6-13	8
β-strand	20	1	14
β-strand	23	1	14
α-helix	26-36	11
β-strand	39	1	13
β-strand	45-46	2	15
β-strand	50-51	2	15
β-strand	52-53	2	16
β-strand	58-59	2	16
β-strand	64	1	17
β-strand	78	1	17
α-helix	81-84	4
α-helix	89-98	10
α-helix	104-107	4
α-helix	109-110	2
α-helix	111-115	5
α-helix	120-124	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Hyhel-10 IgG1 FAB (light chain)	L	protein	214	Mus musculus	P01837 (AlphaFold model)
Hyhel-10 IgG1 FAB (heavy chain)	H	protein	215	Mus musculus
Hen egg white lysozyme	Y	protein	129	Gallus gallus	P00698 (AlphaFold model)

Sequence of entity 1 (L), FASTA

>3HFM_1 HYHEL-10 IGG1 FAB (LIGHT CHAIN) (chains L)
DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPS
RFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIKRADAAPTVSIFPP
SSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLT
LTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Sequence of entity 2 (H), FASTA

>3HFM_2 HYHEL-10 IGG1 FAB (HEAVY CHAIN) (chains H)
DVQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSYSGSTYYN
PSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAAKTTPPS
VYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSS
VTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDC

Sequence of entity 3 (Y), FASTA

>3HFM_3 HEN EGG WHITE LYSOZYME (chains Y)
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
QAWIRGCRL

Primary citation

Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Padlan, E.A., Silverton, E.W., Sheriff, S. et al. Proc Natl Acad Sci U S A (1989) 86:5938-5942. DOI 10.1073/pnas.86.15.5938 · PubMed

Other PDB entries of the same protein (UniProt P01837 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3D9A 1.2 Å, High Resolution Crystal Structure Structure of HyHel10 Fab Complexed to Hen Egg Lysozyme
1RUR 1.5 Å, Crystal Structure (I) of native Diels-Alder antibody 13G5 Fab at pH 8.0 with a data set…
2AJU 1.5 Å, Cyrstal structure of cocaine catalytic antibody 7A1 Fab'
2AJV 1.5 Å, Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with Cocaine
2W60 1.5 Å, Anti citrullinated Collagen type 2 antibody acc4
2W9D 1.57 Å, Structure of Fab fragment of the ICSM 18 - anti-Prp therapeutic antibody at 1.57 A…
3BKJ 1.59 Å, Crystal structure of Fab wo2 bound to the n terminal domain of amyloid beta peptide (1-16)
3BAE 1.59 Å, Crystal structure of Fab WO2 bound to the N terminal domain of Amyloid beta peptide (1-28)
2R1Y 1.6 Å, Crystal structure of S25-2 Fab in complex with Kdo analogues
2R2B 1.6 Å, Crystal structure of S25-2 Fab in complex with Kdo analogues
3BKM 1.6 Å, Structure of anti-amyloid-beta Fab WO2 (Form A, P212121)
3CFB 1.6 Å, High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene…

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3HFM is part of these collections:

Antibodies and the immune system

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