3M1I: Yeast CRM1

Crystal structure of yeast CRM1 (Xpo1p) in complex with yeast RanBP1 (Yrb1p) and yeast RanGTP (Gsp1pGTP). Determined by X-ray diffraction at 2.0 Å resolution. Released 2 Jun 2010.

Method

X-ray diffraction

Resolution

2.0 Å

Organism

Saccharomyces cerevisiae

Chains

3

Atoms

11,634

Mol. weight

167.66 kDa

Ligands

MG, GTP

Released

2 Jun 2010

Explore 3M1I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3M1I contains 82 α-helices and 16 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 7 β-strands

Molecules and chains

>3M1I_1 GTP-binding nuclear protein GSP1/CNR1 (chains A)
MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE
IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV
LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA
SPALAPPEVQVDEQLMQQYQQEMEQATALPLPDEDDADL

>3M1I_2 Ran-specific GTPase-activating protein 1 (chains B)
DKKEEAAPKPPSSAVFSMFGGKKAEKPETKKDEEDTKEETKKEGDDAPESPDIHFEPVVH
LEKVDVKTMEEDEEVLYKVRAKLFRFDADAKEWKERGTGDCKFLKNKKTNKVRILMRRDK
TLKICANHIIAPEYTLKPNVGSDRSWVYACTADIAEGEAEAFTFAIRFGSKENADKFKEE
FEKAQEINKKA

>3M1I_3 Exportin-1 (chains C)
GAMEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFS
TNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKS
DLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAK
ALHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELL
STKFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLK
ATYANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEEREL
FKTTLDYWHNLVADLFYEPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGEIVREF
VKESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSI
SGTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRT
VILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTAD
LQPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSET
VKIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPK
VRGLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCM
TTVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFL
ELPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIF
VSETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYL
ANMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDK
ENALMEQNRLEREKAAKIGGLLKPSELDD

Ligands and cofactors

Primary citation

An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1. Koyama, M., Matsuura, Y. EMBO J (2010) 29:2002-2013. DOI 10.1038/emboj.2010.89 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P32835 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3WYG 2.15 Å, Crystal structure of Xpo1p-PKI-Gsp1p-GTP complex
• 5XOJ 2.2 Å, Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex
• 3WYF 2.22 Å, Crystal structure of Xpo1p-Yrb2p-Gsp1p-GTP complex
• 2X19 2.8 Å, Crystal structure of Importin13 - RanGTP complex
• 9B3I 2.88 Å, Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
• 8QYZ 3.0 Å, Crystal structure of hiNES2 in complex with Xpo1 and RanGTP
• 9D45 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to cargo Pho4 and RanGTP
• 9DZ6 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 3ICQ 3.2 Å, Karyopherin nuclear state
• 9DXM 3.2 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 9OGB 3.25 Å, Cryo-EM structure of human exportin-1 conjugated with selinexor and bound to yeast…
• 8F1E 3.28 Å, Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B

Browse structure collections

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