3M9K: Thioredoxin

Crystal structure of human thioredoxin C69/73S double-mutant, oxidized form. Determined by X-ray diffraction at 1.5 Å resolution. Released 11 Aug 2010.

Method: X-ray diffraction
Resolution: 1.5 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,050
Mol. weight: 23.97 kDa
Ligands: D1D
Released: 11 Aug 2010

Explore 3M9K in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3M9K contains 8 α-helices and 10 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	2-4	3	1
α-helix	8-17	10
β-strand	23-28	6	1
α-helix	33-48	16
β-strand	53-58	6	1
α-helix	63-68	6
β-strand	76-81	6	1
β-strand	84-90	7	1
α-helix	94-104	11

Chain B: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	2-4	3	2
α-helix	8-17	10
β-strand	23-28	6	2
α-helix	33-48	16
β-strand	53-58	6	2
α-helix	68-70	3
β-strand	76-81	6	2
β-strand	84-90	7	2
α-helix	94-104	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thioredoxin	A, B	protein	105	Homo sapiens	P10599 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3M9K_1 Thioredoxin (chains A, B)
MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVD
DCQDVASESEVKSMPTFQFFKKGQKVGEFSGANKEKLEATINELV

Ligands and cofactors

ID	Name	Formula	Copies
D1D	(4S,5S)-1,2-dithiane-4,5-diol	C4 H8 O2 S2	1

Water and common crystallization additives (SO4) are not listed.

Primary citation

Crystal structure of human thioredoxin revealing an unraveled helix and exposed S-nitrosation site. Weichsel, A., Kem, M., Montfort, W.R. Protein Sci (2010) 19:1801-1806. DOI 10.1002/pro.455 · PubMed

Other PDB entries of the same protein (UniProt P10599 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4OO4 0.97 Å, Crystal Structure of Human Thioredoxin Mutant
3M9J 1.1 Å, Crystal structure of human thioredoxin C69/73S double mutant, reduced form
2IFQ 1.2 Å, Crystal structure of S-nitroso thioredoxin
2HSH 1.35 Å, Crystal structure of C73S mutant of human thioredoxin-1 oxidized with H2O2
5DQY 1.4 Å, A fully oxidized human thioredoxin
4OO5 1.54 Å, Crystal Structure of S-nitrosated Human Thioredoxin Mutant
1ERV 1.65 Å, Human thioredoxin mutant with cys 73 replaced by ser (reduced form)
2HXK 1.65 Å, Crystal structure of S-nitroso thioredoxin
1ERT 1.7 Å, Human thioredoxin (reduced form)
2IIY 1.7 Å, Crystal structure of S-nitroso thioredoxin
3KD0 1.7 Å, Human thioredoxin C35S,C62S,C69S,C73S mutant showing cadmium chloride bound to the…
1ERW 1.8 Å, Human thioredoxin double mutant with cys 32 replaced by ser and cys 35 replaced by ser

Browse structure collections

About this viewer

MolViewer shows 3M9K directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.