3NQS: Nitric oxide synthase, inducible

Crystal Structure of Inducible Nitric Oxide Synthase with N-Nitrosated-pterin. Determined by X-ray diffraction at 2.2 Å resolution. Released 21 Jul 2010.

Method
X-ray diffraction
Resolution
2.2 Å
Organism
Mus musculus
Chains
2
Atoms
7,533
Mol. weight
103.6 kDa
Ligands
HEM, H4B, NO, AT2
Released
21 Jul 2010

Explore 3NQS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3NQS contains 58 α-helices and 50 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 25 β-strands

ElementResiduesLengthSheet
β-strand79-8241
β-strand8312
β-strand89-9241
α-helix94-974
α-helix117-1193
β-strand12013
α-helix127-1293
α-helix130-14516
α-helix153-17018
α-helix177-18913
α-helix197-1993
β-strand204-20744
α-helix214-22916
α-helix230-2323
β-strand237-24044
α-helix241-2444
β-strand252-25325
β-strand25714
β-strand26116
β-strand263-26537
β-strand271-27337
α-helix275-2773
α-helix278-2869
α-helix2971
β-strand29816
α-helix299-3002
β-strand301-30445
α-helix308-3103
β-strand311-31335
α-helix314-3163
α-helix317-3193
β-strand322-32438
α-helix331-3366
β-strand339-34138
β-strand345-34624
β-strand350-35349
β-strand356-35839
β-strand363-36424
β-strand367-368210
α-helix369-3702
α-helix371-3766
α-helix377-3782
α-helix386-3927
α-helix400-4023
α-helix404-42219
β-strand427-428210
α-helix430-44819
α-helix455-4584
α-helix464-4663
α-helix468-4714
β-strand47212
β-strand482-48439
β-strand48513
α-helix489-4924
Chain B: 29 helices, 25 β-strands
ElementResiduesLengthSheet
β-strand79-82411
β-strand83112
β-strand89-92411
α-helix94-974
α-helix117-1193
β-strand120113
α-helix127-1293
α-helix130-14617
α-helix153-17018
α-helix177-18913
α-helix197-1993
β-strand204-207414
α-helix214-22916
α-helix230-2323
β-strand237-240414
α-helix241-2444
β-strand252-253215
β-strand257114
β-strand261116
β-strand263-265317
β-strand271-273317
α-helix275-2773
α-helix278-2869
α-helix2971
β-strand298116
α-helix299-3002
β-strand301-304415
α-helix308-3103
β-strand311-313315
α-helix314-3163
α-helix317-3193
β-strand322-324318
α-helix331-3366
β-strand339-341318
β-strand345-346214
β-strand350-353419
β-strand356-358319
β-strand363-364214
β-strand367-368220
α-helix369-3702
α-helix371-3766
α-helix377-3782
α-helix386-3927
α-helix400-4023
α-helix404-42219
β-strand427-428220
α-helix430-44819
α-helix455-4584
α-helix464-4663
α-helix468-4714
β-strand472112
β-strand482-484319
β-strand485113
α-helix489-4924

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Nitric oxide synthase, inducibleA, Bprotein433Mus musculusP29477 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>3NQS_1 Nitric oxide synthase, inducible (chains A, B)
LDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRD
KPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATK
MAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRS
DGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQA
DGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNG
WYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNV
TIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYY
QIEPWKTHIWQNE

Ligands and cofactors

IDNameFormulaCopies
HEMProtoporphyrin IX containing FEC34 H32 Fe N4 O42
H4B5,6,7,8-tetrahydrobiopterinC9 H15 N5 O32
NONitric oxideN O2
AT2Ethyl 4-[(4-methylpyridin-2-yl)amino]piperidine-1-carboxylateC14 H21 N3 O22
BOGoctyl beta-D-glucopyranosideC14 H28 O61

Water and common crystallization additives (GOL, EDO, SO4) are not listed.

Primary citation

Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation. Rosenfeld, R.J., Bonaventura, J., Szymczyna, B.R. et al. J Biol Chem (2010) 285:31581-31589. DOI 10.1074/jbc.M109.072496 · PubMed

Other PDB entries of the same protein (UniProt P29477 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 3NQS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.