3WGV: PDB entry 3WGV

Crystal structure of a Na+-bound Na+,K+-ATPase preceding the E1P state with oligomycin. Determined by X-ray diffraction at 2.8 Å resolution. Released 9 Oct 2013.

Method

X-ray diffraction

Resolution

2.8 Å

Organism

Sus scrofa

Chains

6

Atoms

21,909

Mol. weight

322.98 kDa

Ligands

PC1, CLR, ADP, ALF

Released

9 Oct 2013

Explore 3WGV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3WGV contains 134 α-helices and 102 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 55 helices, 33 β-strands

Molecules and chains

>3WGV_1 Sodium/potassium-transporting ATPase subunit alpha-1 (chains A, C)
GRDKYEPAAVSEHGDKKKAKKERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPAR
AAEILARDGPNALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQN
DNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVV
GDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFST
NCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFF
ILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETL
GSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGL
CNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVEIPFNSTNK
YQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQPLDEELKDAFQNAYLELGG
LGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPLDNLCFVGLISMIDPPRAAVPDAVGKCR
SAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHG
SDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKK
ADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEI
TPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNE
RLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVNWDDRWINDVEDSYGQQWT
YEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAF
LSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

>3WGV_2 Sodium/potassium-transporting ATPase subunit beta-1 (chains B, D)
MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLT
ISEFKPTYQDRVAPPGLTQIPQSQKTEISFRPNDPQSYESYVVSIVRFLEKYKDLAQKDD
MIFEDCGNVPSELKERGEYNNERGERKVCRFRLEWLGNCSGLNDETYGYKDGKPCVIIKL
NRVLGFKPKPPKNESLETYPVMKYNPYVLPVHCTGKRDEDKEKVGTMEYFGLGGYPGFPL
QYYPYYGKLLQPKYLQPLMAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIE
VKS

>3WGV_3 Na+/K+ ATPase gamma subunit transcript variant a (chains E, G)
MAGLSTDDGGSPKGDVDPFYYDYETVRNGGLIFAALAFIVGLIIILSKRLRCGGKKHRPI
NEDEL

Ligands and cofactors

Water and common crystallization additives (NA) are not listed.

Primary citation

Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state. Kanai, R., Ogawa, H., Vilsen, B. et al. Nature (2013) 502:201-206.

Related structures

Other PDB entries of the same protein (UniProt P05024 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3WGU 2.8 Å, Crystal structure of a Na+-bound Na+,K+-ATPase preceding the E1P state without oligomycin
• 8JBK 2.8 Å, Crystal structure of Na+,K+-ATPase in the E1.3Na+ state
• 7WYT 2.9 Å, Crystal structures of Na+,K+-ATPase in complex with ouabain
• 8JBM 2.9 Å, Crystal structure of Na+,K+-ATPase in the E1.Mn2+ state
• 8JBL 3.0 Å, Crystal structure of Na+,K+-ATPase in the E1.Mg2+ state
• 7DDL 3.2 Å, Crystal structures of Na+,K+-ATPase in complex with bufalin
• 7D91 3.35 Å, Crystal Structure of the Na+,K+-ATPase in the E2P state with bound Mg2+ (P4(3)2(1)2…
• 4HYT 3.4 Å, Na,K-ATPase in the E2P state with bound ouabain and Mg2+ in the cation-binding site
• 4RES 3.41 Å, Crystal structure of the Na,K-ATPase E2P-bufalin complex with bound potassium
• 7DDH 3.46 Å, Crystal structures of Na+,K+-ATPase in complex with digoxin
• 3B8E 3.5 Å, Crystal structure of the sodium-potassium pump
• 3KDP 3.5 Å, Crystal structure of the sodium-potassium pump

Browse structure collections

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