3ZNX: OTU domain of OTULIN D336A mutant

Crystal structure of the OTU domain of OTULIN D336A mutant. Determined by X-ray diffraction at 1.35 Å resolution. Released 26 Jun 2013.

Method: X-ray diffraction
Resolution: 1.35 Å
Organism: HOMO SAPIENS
Chains: 1
Atoms: 2,649
Mol. weight: 32.23 kDa
Ligands: CA
Released: 26 Jun 2013

Explore 3ZNX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3ZNX contains 19 α-helices and 11 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	82	1	1
α-helix	83-85	3
β-strand	86-87	2	2
α-helix	88-95	8
α-helix	101-114	14
β-strand	119-121	3	2
α-helix	122	1
β-strand	123	1	1
α-helix	124	1
α-helix	129-140	12
α-helix	147-150	4
α-helix	153-164	12
α-helix	166-170	5
α-helix	171-173	3
α-helix	184-204	21
α-helix	208-218	11
α-helix	223-248	26
α-helix	255-262	8
α-helix	269-275	7
α-helix	277-279	3
β-strand	280	1	3
β-strand	284	1	3
α-helix	288-298	11
β-strand	301-306	6	4
α-helix	307-309	3
α-helix	313-315	3
β-strand	316-319	4	4
β-strand	329-334	6	4
β-strand	340-341	2	4
β-strand	342-344	3	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein FAM105B	A	protein	275	HOMO SAPIENS	Q96BN8 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3ZNX_1 PROTEIN FAM105B (chains A)
GPLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQ
AMSQAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRK
KWAGLAEMRTAEARQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFS
VLLFARDTSNDPGQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFIT
VYPTDPPKDWPVVTLIAEADRHYNIPVRVCEETSL

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1

Water and common crystallization additives (GOL, CL) are not listed.

Primary citation

Otulin Antagonizes Lubac Signaling by Specifically Hydrolyzing met1-Linked Polyubiquitin. Keusekotten, K., Elliott, P.R., Glockner, L. et al. Cell (2013) 153:1312. DOI 10.1016/J.CELL.2013.05.014 · PubMed

Other PDB entries of the same protein (UniProt Q96BN8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3ZNV 1.3 Å, Crystal structure of the OTU domain of OTULIN at 1.3 Angstroms.
4KSJ 1.6 Å, Crystal structure of the OTU domain of Gumby/Fam105B at 1.6 angstrom
6I9C 1.77 Å, Structure of the OTU domain of OTULIN G281R mutant
3ZNZ 1.9 Å, Crystal structure of OTULIN OTU domain (C129A) in complex with Met1- di ubiquitin
6SAK 2.0 Å, Structure of the OTULINcat C129A - SNX27 PDZ domain complex.
9LZX 2.0 Å, Crystal structure of the OTU domain of OTULIN in complex with 11B6
4OYK 2.0 Å, Structure of HOIP PUB domain bound to OTULIN PIM
4KSK 2.4 Å, Gumby/Fam105B in complex with ubiquitin
4P0B 2.7 Å, Crystal structure of HOIP PUB domain in complex with OTULIN PIM
4KSL 2.83 Å, Gumby/Fam105B in complex with linear di-ubiquitin
5OE7 2.95 Å, Structure of OTULIN bound to the Met1-linked diubiquitin activity probe

Browse structure collections

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