4AJX: Insulin
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin. Determined by X-ray diffraction at 1.2 Å resolution. Released 9 Jan 2013.
- Method
- X-ray diffraction
- Resolution
- 1.2 Å
- Organism
- HOMO SAPIENS
- Chains
- 12
- Atoms
- 2,939
- Mol. weight
- 36.18 kDa
- Ligands
- RCO, ZN, 16E
- Released
- 9 Jan 2013
Explore 4AJX in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
4AJX contains 31 α-helices and 12 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A and C: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-6 | 5 | |
| α-helix | 10-12 | 3 | |
| α-helix | 13-16 | 4 | |
| α-helix | 17-19 | 3 | |
| β-strand | 20 | 1 | 1 |
Chains B, F, H and L: 2 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-19 | 18 | |
| α-helix | 20-22 | 3 | |
| β-strand | 24-26 | 3 | 1 |
Chain D: 1 helix, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-18 | 16 | |
| β-strand | 24-26 | 3 | 2 |
Chains E and G: 3 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-8 | 7 | |
| α-helix | 10-12 | 3 | |
| α-helix | 13-17 | 5 | |
| β-strand | 20 | 1 | 3 |
Chain I: 3 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-6 | 5 | |
| α-helix | 10-12 | 3 | |
| α-helix | 13-17 | 5 | |
| β-strand | 20 | 1 | 3 |
Chain J: 2 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-19 | 17 | |
| α-helix | 20-22 | 3 | |
| β-strand | 24-26 | 3 | 3 |
Chain K: 3 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-8 | 7 | |
| α-helix | 13-16 | 4 | |
| α-helix | 17-19 | 3 | |
| β-strand | 20 | 1 | 2 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Insulin | A, C, E, G, I, K | protein | 21 | HOMO SAPIENS | P01308 (AlphaFold model) |
| Insulin | B, D, F, H, J, L | protein | 29 | HOMO SAPIENS | P01308 (AlphaFold model) |
Sequence of entity 1 (A, C, E, G, I, K), FASTA
>4AJX_1 INSULIN (chains A, C, E, G, I, K)
GIVEQCCTSICSLYQLENYCN
Sequence of entity 2 (B, D, F, H, J, L), FASTA
>4AJX_2 INSULIN (chains B, D, F, H, J, L)
FVNQHLCGSHLVEALYLVCGERGFFYTPK
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| RCO | Resorcinol | C6 H6 O2 | 6 |
| ZN | Zinc ion | Zn | 2 |
| 16E | N-(16-Carboxyhexadecanoyl)-L-glutamic acid | C21 H37 N O7 | 2 |
Water and common crystallization additives (IMD, NA) are not listed.
Primary citation
Ligand Controlled Assembly of Hexamers, Dihexamers, and Linear Multihexamer Structures by the Engineered Acylated Insulin Degludec. Steensgaard, D.B., Schluckebier, G., Strauss, H.M. et al. Biochemistry (2013) 52:295. DOI 10.1021/BI3008609 · PubMed
Other PDB entries of the same protein (UniProt P01308 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 3W7Y 0.92 Å, 0.92A structure of 2Zn human insulin at 100K
- 5E7W 0.95 Å, X-ray Structure of Human Recombinant 2Zn insulin at 0.92 Angstrom
- 5HQI 0.97 Å, Insulin with proline analog HzP at position B28 in the T2 state
- 1MSO 1.0 Å, T6 Human Insulin at 1.0 A Resolution
- 3HYD 1.0 Å, LVEALYL peptide derived from human insulin chain B, residues 11-17
- 6VER 1.05 Å, Human insulin analog: [GluB10,TyrB20]-DOI
- 4FKA 1.08 Å, High resolution structure of the manganese derivative of insulin
- 3TT8 1.12 Å, Crystal Structure Analysis of Cu Human Insulin Derivative
- 3W7Z 1.15 Å, 1.15A structure of human 2Zn insulin at 293K
- 8OKY 1.17 Å, Crystal structure of D-ProB26-DTriA analogue of human insulin
- 5USP 1.17 Å, Insulin with proline analog Pip at position B28 in the T2 state
- 5UOZ 1.17 Å, Insulin with proline analog FyP at position B28 in the T2 state
Browse structure collections
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