Crystal structure of the human topoisomerase III alpha-RMI1 complex with bound calcium ion. Determined by X-ray diffraction at 3.25 Å resolution. Released 12 Feb 2014.
Explore 4CHT in 3D Show helices and sheets RCSB PDB PDBe
4CHT contains 43 α-helices and 30 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 23-30 | 8 | |
| β-strand | 35-40 | 6 | 1 |
| α-helix | 43-54 | 12 | |
| β-strand | 59-61 | 3 | 1 |
| β-strand | 70-77 | 8 | 1 |
| β-strand | 80-88 | 9 | 1 |
| β-strand | 95-98 | 4 | 2 |
| α-helix | 100-103 | 4 | |
| α-helix | 105-107 | 3 | |
| α-helix | 110-114 | 5 | |
| β-strand | 118-121 | 4 | 2 |
| α-helix | 124-126 | 3 | |
| α-helix | 127-139 | 13 | |
| β-strand | 142-146 | 5 | 1 |
| α-helix | 151-165 | 15 | |
| β-strand | 172-175 | 4 | 1 |
| α-helix | 183-191 | 9 | |
| β-strand | 194 | 1 | 1 |
| α-helix | 198-229 | 32 | |
| α-helix | 231-234 | 4 | |
| α-helix | 245-261 | 17 | |
| β-strand | 266-276 | 11 | 3 |
| β-strand | 279-285 | 7 | 3 |
| β-strand | 290 | 1 | 3 |
| α-helix | 293-304 | 12 | |
| β-strand | 309-321 | 13 | 3 |
| α-helix | 322-325 | 4 | |
| α-helix | 329-335 | 7 | |
| α-helix | 336-340 | 5 | |
| α-helix | 344-356 | 13 | |
| β-strand | 360 | 1 | 4 |
| α-helix | 376-381 | 6 | |
| α-helix | 390-398 | 9 | |
| β-strand | 419 | 1 | 4 |
| α-helix | 428-444 | 17 | |
| β-strand | 449-460 | 12 | 3 |
| β-strand | 463-474 | 12 | 3 |
| α-helix | 476-479 | 4 | |
| β-strand | 489 | 1 | 3 |
| α-helix | 490-493 | 4 | |
| β-strand | 499-500 | 2 | 3 |
| β-strand | 504-511 | 8 | 3 |
| α-helix | 512-515 | 4 | |
| α-helix | 517-519 | 3 | |
| α-helix | 520-529 | 10 | |
| α-helix | 538-547 | 10 | |
| β-strand | 551-553 | 3 | 5 |
| β-strand | 559-561 | 3 | 5 |
| α-helix | 563-573 | 11 | |
| α-helix | 583-597 | 15 | |
| α-helix | 602-622 | 21 | |
| α-helix | 625-635 | 11 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 4-18 | 15 | |
| α-helix | 24-37 | 14 | |
| α-helix | 45-58 | 14 | |
| α-helix | 61-63 | 3 | |
| α-helix | 70 | 1 | |
| β-strand | 78-81 | 4 | 6 |
| β-strand | 85-94 | 10 | 6 |
| α-helix | 99-106 | 8 | |
| α-helix | 113-115 | 3 | |
| α-helix | 126-129 | 4 | |
| α-helix | 131-133 | 3 | |
| β-strand | 135-140 | 6 | 6 |
| β-strand | 145-150 | 6 | 6 |
| α-helix | 162 | 1 | |
| β-strand | 166-169 | 4 | 6 |
| β-strand | 173-176 | 4 | 6 |
| β-strand | 179-182 | 4 | 6 |
| α-helix | 184-186 | 3 | |
| β-strand | 187-191 | 5 | 6 |
| α-helix | 195-200 | 6 | |
| α-helix | 203-209 | 7 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| DNA topoisomerase 3-alpha | A | protein | 755 | HOMO SAPIENS | Q13472 (AlphaFold model) |
| Recq-mediated genome instability protein 1 | B | protein | 219 | HOMO SAPIENS | Q9H9A7 (AlphaFold model) |
>4CHT_1 DNA TOPOISOMERASE 3-ALPHA (chains A) GGRIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRM RRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEI EKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARF SEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQL ISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLV LYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQG YISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIH PTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYL DVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATH AEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDG KKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKC PQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKL KFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFS
>4CHT_2 RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 (chains B) MNVTSIALRAETWLLAAWHVKVPPMWLEACINWIQEENNNVNLSQAQMNKQVFEQWLLTD LRDLEHPLLPDGILEIPKGELNGFYALQINSLVDVSQPAYSQIQKLRGKNTTNDLVTAEA QVTPKPWEAKPSRMLMLQLTDGIVQIQGMEYQPIPILHSDLPPGTKILIYGNISFRLGVL LLKPENVKVLGGEVDALLEEYAQEKVLARLIGEPDLVVS
| ID | Name | Formula | Copies |
|---|---|---|---|
| CA | Calcium ion | Ca | 1 |
Structural and Mechanistic Insight Into Holliday-Junction Dissolution by Topoisomerase Iiialpha and Rmi1. Bocquet, N., Bizard, A.H., Abdulrahman, W. et al. Nat Struct Mol Biol (2014) 21:261. DOI 10.1038/NSMB.2775 · PubMed
Other PDB entries of the same protein (UniProt Q13472 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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