4K4S: Poliovirus polymerase elongation complex

Poliovirus polymerase elongation complex (r3_form). Determined by X-ray diffraction at 2.4 Å resolution. Released 22 May 2013.

Method
X-ray diffraction
Resolution
2.4 Å
Organism
Human poliovirus 1
Chains
8
Atoms
9,103
Mol. weight
137.11 kDa
Ligands
ZN
Released
22 May 2013

Explore 4K4S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4K4S contains 65 α-helices and 44 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 32 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand2-871
α-helix9-113
α-helix14-163
β-strand26-2722
β-strand38-4033
α-helix41-422
α-helix54-596
α-helix72-8615
α-helix94-963
α-helix97-1026
β-strand10414
β-strand10714
α-helix108-1103
α-helix120-1234
α-helix127-1293
α-helix139-14810
α-helix152-1532
β-strand154-15851
β-strand162-16433
α-helix165-1706
β-strand175-17841
α-helix179-1802
α-helix181-20020
β-strand20315
β-strand20815
α-helix214-2174
α-helix221-2244
β-strand228-23035
β-strand233-23426
α-helix237-2404
α-helix243-25614
α-helix259-2613
α-helix263-2697
β-strand270-27561
β-strand278-28361
α-helix293-31220
α-helix318-3203
β-strand322-32655
β-strand329-33465
α-helix340-34910
β-strand354-35526
α-helix357-3593
β-strand37317
β-strand37617
β-strand377-38048
β-strand388-39148
α-helix394-4018
β-strand403-40422
α-helix407-4093
α-helix410-42112
α-helix422-4243
α-helix426-43611
α-helix440-4445
α-helix450-45910
Chain E: 33 helices, 22 β-strands
ElementResiduesLengthSheet
β-strand2-879
α-helix9-113
α-helix14-163
α-helix18-203
β-strand26-27210
β-strand38-40311
α-helix41-422
α-helix54-596
α-helix72-8615
α-helix94-963
α-helix97-1026
β-strand104112
β-strand107112
α-helix108-1103
α-helix120-1234
α-helix127-1293
α-helix139-14810
α-helix152-1532
β-strand154-15859
β-strand162-164311
α-helix165-1706
β-strand175-17849
α-helix179-1802
α-helix181-20020
β-strand203113
β-strand208113
α-helix214-2174
α-helix221-2244
β-strand228-230313
β-strand233-234214
α-helix237-2404
α-helix243-25513
α-helix259-2613
α-helix263-2697
β-strand270-27569
β-strand278-28369
α-helix293-31220
α-helix318-3203
β-strand322-326513
β-strand329-334613
α-helix340-34910
β-strand354-355214
α-helix357-3593
β-strand373115
β-strand376115
β-strand377-380416
β-strand388-391416
α-helix394-4018
β-strand403-404210
α-helix407-4093
α-helix410-42112
α-helix422-4243
α-helix426-43611
α-helix442-4443
α-helix450-45910

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
RNA-directed RNA polymerase 3D-POLA, Eprotein471Human poliovirus 1P03300 (AlphaFold model)
RNA (5'-r(*ap*ap*gp*up*cp*up*cp*cp*ap*gp*gp*up*cp*up*cp*up*cp*gp*cp*gp*ap*ap*a)-3')B, FRNA23
RNA (5'-r(*up*gp*up*up*cp*gp*cp*gp*ap*gp*ap*gp*a)-3')C, GRNA13
RNA (5'-r(*gp*gp*gp*ap*gp*ap*up*gp*a)-3')D, HRNA9
Sequence of entity 1 (A, E), FASTA
>4K4S_1 RNA-directed RNA polymerase 3D-POL (chains A, E)
GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRLKTDFEEAIFS
KYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDAMYGTDGLEALDLSTSAGYPY
VAMGKKKRDILNKQTRDTKEMQKLLDTYGINLPLVTYVKDELRSKTKVEQGKSRLIEASS
LNDSVAMRMAFGNLYAAFHKNPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDAS
LSPAWFEALKMVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDYGLTMTPADKS
ATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHESIRWTKDPRNTQDHVRSLCLL
AWHNGEEEYNKFLAKIRSVPIGRALDLPEYSTLYRRWLDSFGSSSHHHHHH
Sequence of entity 2 (B, F), FASTA
>4K4S_2 RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*CP*GP*AP*AP*A)-3') (chains B, F)
AAGUCUCCAGGUCUCUCGCGAAA
Sequence of entity 3 (C, G), FASTA
>4K4S_3 RNA (5'-R(*UP*GP*UP*UP*CP*GP*CP*GP*AP*GP*AP*GP*A)-3') (chains C, G)
UGUUCGCGAGAGA
Sequence of entity 4 (D, H), FASTA
>4K4S_4 RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3') (chains D, H)
GGGAGAUGA

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn2

Water and common crystallization additives (GOL) are not listed.

Primary citation

Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts. Gong, P., Kortus, M.G., Nix, J.C. et al. PLoS One (2013) 8:e60272-e60272. DOI 10.1371/journal.pone.0060272 · PubMed

Other PDB entries of the same protein (UniProt P03300 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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